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Plasmid#25712PurposeBacterial expression of Taq polymeraseDepositorInsertTaq polymerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO-TtAgocatdead
Plasmid#210322PurposeBacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilusDepositorInsertTtAgo (TT_P0026 Thermus thermophilus)
UseTags6x His tag and SUMO tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTT610
Plasmid#101742Purposeencoding SecY(L2V, R252G)–(GGSG)4–SecA1-939–His10 fusion protein and SecE and SecG from Thermus thermophilus HB8DepositorUseTagsHis10ExpressionBacterialMutationSecY(L2V, R252G)–(GGSG)4–SecA1-939–His10 fusion p…PromoterLacAvailable sinceNov. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCDF-5xT7-TtCsm
Plasmid#128572PurposeFor expression of T. thermophilus Csm in E. coli (Csm1-5)DepositorInsertsCsm1
Csm2, Csm3, Csm4, Csm5
UseTagsHRV 3C protease cleavage site and 10xHis tag on C…ExpressionBacterialMutationCodon optimized for expression in E. coli and Cod…PromoterT7 promoter, lac operatorAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSKB2-TRex
Plasmid#31349DepositorInsertThermus aquaticus Redox Sensing Repressor
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
OAH2
Plasmid#64074PurposeExpression of O-acetyl-L-homoserine sulfhydrylase (OAH2) of Thermus thermophilusDepositorInsertO-acetyl-L-homoserine sulfhydrylase
UseTagsnoneExpressionBacterialMutationPromoterAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAK22
Plasmid#101741Purposeencoding two copies of T. thermophilus SecG for expression of SecYEG complexDepositorInsertThermus thermophilus HB8 SecG x 2
UseTagsNONEExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI
Plasmid#115270PurposeExpresses TtCsm6 from a SUMO-tagged bacterial expression constructDepositorInsertTtCsm6
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO-TtAgo
Plasmid#210191PurposeBacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilusDepositorInsertTtAgo (TT_P0026 Thermus thermophilus)
UseTags6x His tag and SUMO tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSKB2-TRexR90D
Plasmid#31350DepositorInsertThermus aquaticus Redox Sensing Repressor (R90D)
UseTagsHisExpressionBacterialMutationchanged Arginine 90 to Aspartic AcidPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pETMutS
Plasmid#13245DepositorInsertMutS
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-28a_6H-TAQ_E602D
Plasmid#166944PurposeBacterial expression of Taq DNA polymeraseDepositorInsertThermus aquaticus DNA polymerase
UseTags6X HisExpressionBacterialMutationE602DPromoterT7Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTTCC
Plasmid#164264PurposeExpresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilusDepositorInsertsCas9
gRNA scaffold
UseTagsExpressionBacterialMutationPromoterPcel and trcAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-IDI2
Plasmid#108951Purposeheterologous expression of IDI2 of T. thermophilus in E. coliDepositorInsertIsopentenyldiphosphat isomerase 2 (IDI2)
UseTagsHis6-TagExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Taq
Plasmid#102793PurposeExpresses Taq DNA polymerase under WT T7 promoterDepositorInsertTaq DNA polymerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-LipB
Plasmid#30165DepositorInsertlipoate protein ligase B
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAtetO_6xHis-Klentaq
Plasmid#157862PurposePlasmid for the aTc-inducible expression of the KlentaqDepositorInsertKlentaq
UseTags6xHisExpressionBacterialMutationPromotertet PAAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYC.Taq.1Amb
Plasmid#102794PurposeExpresses Taq DNA polymerases with a premature stop codonDepositorInsertTaq DNA polymerase
UseTagsExpressionBacterialMutationW168Stop; *(see below)PromoterAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETMutS-E41A
Plasmid#13048DepositorInsertMutS
UseTagsExpressionBacterialMutationmutation Glu41 to Ala full-lengthPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only