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Addgene

pET-28a(+)-IDI2
(Plasmid #108951)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 108951 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28a(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Isopentenyldiphosphat isomerase 2 (IDI2)
  • Species
    Thermus thermophilus
  • Tag / Fusion Protein
    • His6-Tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

codon-optimized synthetic gene for expression in E. coli

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28a(+)-IDI2 was a gift from Frank Schulz (Addgene plasmid # 108951 ; http://n2t.net/addgene:108951 ; RRID:Addgene_108951)

  • For your References section:

    An in vitro Biosynthesis of Sesquiterpenes starting from Acetic Acid. Schulz F, Dirkmann M, Nowack J. Chembiochem. 2018 Aug 7. doi: 10.1002/cbic.201800128. 10.1002/cbic.201800128 PubMed 30085399
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