Skip to main content
Addgene

pETMutS-E41A
(Plasmid #13048)

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13048 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET3a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4640
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MutS
  • Species
    Thermus aquaticus
  • Insert Size (bp)
    3000
  • Mutation
    mutation Glu41 to Ala full-length
  • GenBank ID
    U33117

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 fwd
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETMutS-E41A was a gift from Peggy Hsieh (Addgene plasmid # 13048 ; http://n2t.net/addgene:13048 ; RRID:Addgene_13048)
  • For your References section:

    The Phe-X-Glu DNA binding motif of MutS. The role of hydrogen bonding in mismatch recognition. Schofield MJ, Brownewell FE, Nayak S, Du C, Kool ET, Hsieh P. J Biol Chem. 2001 Dec 7. 276(49):45505-8. 10.1074/jbc.C100449200 PubMed 11602569