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Addgene

pETMutS
(Plasmid #13245)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13245 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET3a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4640
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21 (DE3) cells for expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MutS
  • Species
    T. aquaticus
  • Insert Size (bp)
    3396
  • Mutation
    None
  • GenBank ID
    U33117

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 fwd
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETMutS was a gift from Peggy Hsieh (Addgene plasmid # 13245 ; http://n2t.net/addgene:13245 ; RRID:Addgene_13245)
  • For your References section:

    Identification and characterization of a thermostable MutS homolog from Thermus aquaticus. Biswas I, Hsieh P. J Biol Chem 1996 Mar 1;271(9):5040-8. 10.1074/jbc.271.9.5040 PubMed 8617781