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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13245 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET3a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 4640
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsUse BL21 (DE3) cells for expression.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMutS
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SpeciesT. aquaticus
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Insert Size (bp)3396
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MutationNone
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GenBank IDU33117
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 fwd (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETMutS was a gift from Peggy Hsieh (Addgene plasmid # 13245 ; http://n2t.net/addgene:13245 ; RRID:Addgene_13245) -
For your References section:
Identification and characterization of a thermostable MutS homolog from Thermus aquaticus. Biswas I, Hsieh P. J Biol Chem 1996 Mar 1;271(9):5040-8. 10.1074/jbc.271.9.5040 PubMed 8617781