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PurposeBacterial expression of Taq polymerase
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 25712 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTTQ18
- Backbone size w/o insert (bp) 4563
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTaq polymerase
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SpeciesThermus aquaticus
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Insert Size (bp)2501
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BglII (unknown if destroyed)
- 5′ sequencing primer Tac promoter
- 3′ sequencing primer M13_puc_F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Addgene Notes
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAKTaq was a gift from David Engelke (Addgene plasmid # 25712 ; http://n2t.net/addgene:25712 ; RRID:Addgene_25712) -
For your References section:
Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli. Engelke DR, Krikos A, Bruck ME, Ginsburg D. Anal Biochem. 1990 Dec . 191(2):396-400. 10.1016/0003-2697(90)90238-5 PubMed 2085185