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PurposeHigh copy number plasmid 1 to prepare Penn State DNA molecular weight ladders
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 89439 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC9
- Backbone size w/o insert (bp) 2635
- Total vector size (bp) 10000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert name500 bp EcoRV fragment
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Alt namelambda DNA position 650-1149
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Speciesphage lambda
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Insert Size (bp)500
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GenBank IDNC_001416.1
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site HIndIII (not destroyed)
- 3′ cloning site None (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert name1000 bp EcoRV fragment
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Alt namelambda DNA position 6681-7681
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Speciesphage lambda
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Insert Size (bp)1000
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GenBank IDNC_001416.1
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site None (not destroyed)
- 3′ cloning site None (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Gene/Insert 3
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Gene/Insert name1500 bp EcoRV fragment
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Alt namelambda DNA position 28223-29710
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Speciesphage lambda
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Insert Size (bp)1500
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GenBank IDNC_001416.1
Cloning Information for Gene/Insert 3
- Cloning method Restriction Enzyme
- 5′ cloning site None (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Gene/Insert 4
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Gene/Insert name2000 bp EcoRV fragment
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Alt namelambda DNA position 36060-37994
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Speciesphage lambda
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Insert Size (bp)2000
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GenBank IDNC_001416.1
Cloning Information for Gene/Insert 4
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Gene/Insert 5
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Gene/Insert name500 bp PstI fragment
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Alt namelambda DNA position 28223-28663
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Speciesphage lambda
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Insert Size (bp)500
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GenBank IDNC_001416.1
Gene/Insert 6
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Gene/Insert name700 bp PstI fragment
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Alt namelambda DNA position 8783-9468
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Speciesphage lambda
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Insert Size (bp)700
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GenBank IDNC_001416.1
Cloning Information for Gene/Insert 6
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Gene/Insert 7
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Gene/Insert name800 bp PstI fragment
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Alt nameG9a position 2757-3535
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Alt nameEHMT2
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SpeciesH. sapiens (human)
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Insert Size (bp)800
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GenBank IDNM_006709.4
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Entrez GeneEHMT2 (a.k.a. BAT8, C6orf30, G9A, GAT8, KMT1C, NG36)
Cloning Information for Gene/Insert 7
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site BamHI (destroyed during cloning)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Gene/Insert 8
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Gene/Insert name900 bp PstI fragment
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Alt namelambda DNA position 143381-15280
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Speciesphage lambda
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Insert Size (bp)900
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GenBank IDNC_001416.1
Cloning Information for Gene/Insert 8
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Gene/Insert 9
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Gene/Insert name1000 bp PstI fragment
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Alt nameBmi1 position 5-979
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SpeciesH. sapiens (human)
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Insert Size (bp)1000
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GenBank IDNM_005180.8
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Entrez GeneBMI1 (a.k.a. FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1)
Gene/Insert 10
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Gene/Insert name2000 bp PstI fragment
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Alt namelambda DNA position 28670-29710 and 36060-37994
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Speciesphage lambda
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Insert Size (bp)2000
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GenBank IDNC_001416.1
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPSU1 was a gift from Song Tan (Addgene plasmid # 89439 ; http://n2t.net/addgene:89439 ; RRID:Addgene_89439) -
For your References section:
The pPSU Plasmids for Generating DNA Molecular Weight Markers. Henrici RC, Pecen TJ, Johnston JL, Tan S. Sci Rep. 2017 May 26;7(1):2438. doi: 10.1038/s41598-017-02693-1. 10.1038/s41598-017-02693-1 [pii] PubMed 28550309