pACYC.Taq.1Amb
(Plasmid
#102794)
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PurposeExpresses Taq DNA polymerases with a premature stop codon
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102794 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACYC
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Backbone manufacturerNovagen
- Total vector size (bp) 6169
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTaq DNA polymerase
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SpeciesThermus aquaticus
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Insert Size (bp)2502
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MutationW168Stop; *(see below)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
*Note: Addgene's quality control sequencing finds an additional V155A amino acid residue substitution. This residue change should not affect protein function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACYC.Taq.1Amb was a gift from Andrew Ellington (Addgene plasmid # 102794 ; http://n2t.net/addgene:102794 ; RRID:Addgene_102794) -
For your References section:
Compartmentalized partnered replication for the directed evolution of genetic parts and circuits. Abil Z, Ellefson JW, Gollihar JD, Watkins E, Ellington AD. Nat Protoc. 2017 Dec;12(12):2493-2512. doi: 10.1038/nprot.2017.119. Epub 2017 Nov 9. 10.1038/nprot.2017.119 PubMed 29120463