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Compartmentalized partnered replication for the directed evolution of genetic parts and circuits.

Abil Z, Ellefson JW, Gollihar JD, Watkins E, Ellington AD
Nat Protoc. 2017 Dec;12(12):2493-2512. doi: 10.1038/nprot.2017.119. Epub 2017 Nov 9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
102789pRST11B-AS3_4Expresses tryptophanyl tRNA synthetase (ScWRS) and supressor tryptophanyl tRNA
102790pQE 80L Kan T7WTExpresses T7 RNA polymerase
102791pACYC-GFPM2Expresses GFP under WT T7 promoter
102792pACYC.GFP1AmbExpresses GFP with a premature stop codon
102793pACYC-TaqExpresses Taq DNA polymerase under WT T7 promoter
102794pACYC.Taq.1AmbExpresses Taq DNA polymerases with a premature stop codon

Antibodies from Article