pRST11B-AS3_4
(Plasmid
#102789)
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PurposeExpresses tryptophanyl tRNA synthetase (ScWRS) and supressor tryptophanyl tRNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 102789 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepRST11B
- Total vector size (bp) 6434
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nametryptophanyl tRNA synthetase
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Alt nameScWRS
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1299
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Entrez GeneWRS1 (a.k.a. YOL097C, HRE342)
Gene/Insert 2
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Gene/Insert namesuppressor tRNA
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Insert Size (bp)100
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRST11B-AS3_4 was a gift from Andrew Ellington (Addgene plasmid # 102789 ; http://n2t.net/addgene:102789 ; RRID:Addgene_102789) -
For your References section:
Compartmentalized partnered replication for the directed evolution of genetic parts and circuits. Abil Z, Ellefson JW, Gollihar JD, Watkins E, Ellington AD. Nat Protoc. 2017 Dec;12(12):2493-2512. doi: 10.1038/nprot.2017.119. Epub 2017 Nov 9. 10.1038/nprot.2017.119 PubMed 29120463