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Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6.

Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F
Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
115207pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaIExpresses BzoCas13b from a SUMO-tagged bacterial expression construct
115208pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaIExpresses PinCas13b from a SUMO-tagged bacterial expression construct
115209pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaIExpresses PbuCas13b from a SUMO-tagged bacterial expression construct
115210pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaIExpresses AspCas13b from a SUMO-tagged bacterial expression construct
115211pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaIExpresses PsmCas13b from a SUMO-tagged bacterial expression construct
115213pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaIExpresses RanCas13b from a SUMO-tagged bacterial expression construct
115214pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaIExpresses PauCas13b from a SUMO-tagged bacterial expression construct
115216pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaIExpresses Pin2Cas13b from a SUMO-tagged bacterial expression construct
115218pC0067 PguCas13 (B11) His6-TwinStrep-SUMO-BsaIExpresses PguCas13b from a SUMO-tagged bacterial expression construct
115219pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaIExpresses PspCas13b from a SUMO-tagged bacterial expression construct
115220pC0070 Pin3Cas13 (B15) His6-TwinStrep-SUMO-BsaIExpresses Pin3Cas13b from a SUMO-tagged bacterial expression construct
115267pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaIExpresses LbuCas13a from a SUMO-tagged bacterial expression construct
115268pC0073 EiCsm6 His6-TwinStrep-SUMO-BsaIExpresses EiCsm6 from a SUMO-tagged bacterial expression construct
115270pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaIExpresses TtCsm6 from a SUMO-tagged bacterial expression construct
182687pR0306 CcaCas13b His6-TwinStrep-SUMOExpresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purification

Antibodies from Article