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Addgene

pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI
(Plasmid #115214)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115214 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    SUMO bacterial expression vector
  • Backbone size w/o insert (bp) 6087
  • Total vector size (bp) 9459
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PauCas13
  • Alt name
    Cas13a from Prevotella aurantiaca
  • Species
    Prevotella aurantiaca
  • Insert Size (bp)
    3372
  • Tag / Fusion Protein
    • SUMO

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PauCas13 contains a K863M difference compared to the NCBI reference that does not impact function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pC0063 PauCas13 (B07) His6-TwinStrep-SUMO-BsaI was a gift from Feng Zhang (Addgene plasmid # 115214 ; http://n2t.net/addgene:115214 ; RRID:Addgene_115214)

  • For your References section:

    Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F. Science. 2018 Apr 27;360(6387):439-444. doi: 10.1126/science.aaq0179. Epub 2018 Feb 15. 10.1126/science.aaq0179 PubMed 29449508
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