pSKB2-TRex
(Plasmid
#31349)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31349 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSKB2 (pET28b derived)
- Backbone size w/o insert (bp) 5368
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameThermus aquaticus Redox Sensing Repressor
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Alt nameRex
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Alt namep25
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Alt nameT-Rex
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SpeciesThermus Aquaticus
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Insert Size (bp)1139
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GenBank IDAF061257
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSKB2-TRex was a gift from Clara Kielkopf (Addgene plasmid # 31349 ; http://n2t.net/addgene:31349 ; RRID:Addgene_31349) -
For your References section:
Structural basis for NADH/NAD+ redox sensing by a Rex family repressor. McLaughlin KJ, Strain-Damerell CM, Xie K, Brekasis D, Soares AS, Paget MS, Kielkopf CL. Mol Cell. 2010 May 28. 38(4):563-75. 10.1016/j.molcel.2010.05.006 PubMed 20513431