pETSUMO-TtAgo
(Plasmid
#210191)
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PurposeBacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 210191 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepETSUMO
- Backbone size w/o insert (bp) 5643
- Total vector size (bp) 7701
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTtAgo
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Alt nameTT_P0026
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SpeciesThermus thermophilus
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Insert Size (bp)2057
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Entrez GeneTT_P0026 (a.k.a. TT_P0026, TTP0026)
- Promoter T7
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Tags
/ Fusion Proteins
- 6x His tag (N terminal on backbone)
- SUMO tag (N terminal on backbone)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer SUMO Forward
- 3′ sequencing primer T7 Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETSUMO-TtAgo was a gift from Phillip Zamore (Addgene plasmid # 210191 ; http://n2t.net/addgene:210191 ; RRID:Addgene_210191) -
For your References section:
An automated Bayesian pipeline for rapid analysis of single-molecule binding data. Smith CS, Jouravleva K, Huisman M, Jolly SM, Zamore PD, Grunwald D. Nat Commun. 2019 Jan 17;10(1):272. doi: 10.1038/s41467-018-08045-5. 10.1038/s41467-018-08045-5 PubMed 30655518