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Showing: 1 - 50 of 72 results

  1. pGLU_111

    Plasmid
    #225792
    Purpose
    RS421 library variant 13463
    Depositor
    Nili Ostrov
    Insert
    13463_[Tn5]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225792-plasmid-map-sequence-id-447013

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  2. pGLU_75

    Plasmid
    #225777
    Purpose
    RS421 library variant 13463
    Depositor
    Nili Ostrov
    Insert
    13463_[abr]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225777-plasmid-map-sequence-id-446990

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  3. pGLU_93

    Plasmid
    #225807
    Purpose
    RS421 library variant 13463
    Depositor
    Nili Ostrov
    Insert
    13463_[Himar]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    N/A
    Promoter
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    225807-plasmid-map-sequence-id-446975

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  4. pRL443

    Plasmid
    #70261
    Purpose
    Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.
    Depositor
    Peter Wolk
    Article
    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70261-plasmid-map-sequence-id-348706

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  5. pRL277

    Plasmid
    #70255
    Purpose
    sacB plasmid with polylinker; streptomycin/spectinomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.
    Depositor
    Peter Wolk
    Article
    Cai et al J Bacteriol. 1990 Jun;172(6):3138-45.
    Type
    Empty backbone
    Use
    Conditionally lethal
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    70255-plasmid-map-sequence-id-125552

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  6. pRL271

    Plasmid
    #70254
    Purpose
    sacB plasmid with polylinker; chloramphenicol/erythromycin resistance: use as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.
    Depositor
    Peter Wolk
    Article
    Cai et al J Bacteriol. 1990 Jun;172(6):3138-45.
    Type
    Empty backbone
    Use
    Conditionally lethal
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70254-plasmid-map-sequence-id-125549

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  7. pRL623

    Plasmid
    #58494
    Purpose
    helper plasmid for bacterial conjugal DNA transfer
    Depositor
    Peter Wolk
    Article
    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.
    Insert
    encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    58494-plasmid-map-sequence-id-420569

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  8. pRL278

    Plasmid
    #70256
    Purpose
    sacB plasmid with polylinker; kanamycin/neomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.
    Depositor
    Peter Wolk
    Article
    Cai et al J Bacteriol. 1990 Jun;172(6):3138-45.
    Type
    Empty backbone
    Use
    Conditionally lethal
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    70256-plasmid-map-sequence-id-125559

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  9. pRL25

    Plasmid
    #70253
    Purpose
    Shuttle vector suitable for transfer to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains
    Depositor
    Peter Wolk
    Article
    Wolk et al J Bacteriol. 1988 Mar;170(3):1239-44.
    Type
    Empty backbone
    Use
    Bacterial shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70253-plasmid-map-sequence-id-457912

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  10. pRL1383a

    Plasmid
    #70692
    Purpose
    RSF1010-based vector with extensive polylinker, and resistance to Strep and Spec. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.
    Depositor
    Peter Wolk
    Article
    Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.
    Type
    Empty backbone
    Use
    Bacterial shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    70692-plasmid-map-sequence-id-126476

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  11. pRL1124

    Plasmid
    #70690
    Purpose
    provides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.
    Depositor
    Peter Wolk
    Article
    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.
    Inserts
    avaiM
    eco47iiM
    ecot22iM
    Use
    Bacterial shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    70690-plasmid-map-sequence-id-126465

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  12. pRL1342

    Plasmid
    #70691
    Purpose
    RSF1010-based vector with extensive polylinker, and resistance to Cm and Em. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.
    Depositor
    Peter Wolk
    Article
    Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.
    Type
    Empty backbone
    Use
    Bacterial shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    70691-plasmid-map-sequence-id-126468

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  17. Ana GvpC-SpyTag-His in pET28a

    Plasmid
    #85737
    Purpose
    Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi
    Insert
    Gas vesicle protein C (fused to SpyTag and His-tagged)
    Use
    Tags
    His-tag and SpyTag
    Expression
    Bacterial
    Mutation
    Added an extra glycine after the methionine at th…
    Promoter
    T7
    Available since
    March 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    85737-plasmid-map-sequence-id-173841

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  22. N-His-GvpC-ssrA

    Plasmid
    #153297
    Purpose
    Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d
    Insert
    Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
    Use
    Tags
    His-tag and ssrA tag
    Expression
    Bacterial
    Mutation
    1) N-terminal 6xHis tag flanked by 1x glycine res…
    Promoter
    T7
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153297-plasmid-map-sequence-id-310693

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  23. delNC C-His Ana GvpC in pET28a

    Plasmid
    #85736
    Purpose
    Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi
    Insert
    Gas vesicle protein C (truncated and his-tagged)
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Deletion of N and C terminal regions, addition of…
    Promoter
    T7
    Available since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    85736-plasmid-map-sequence-id-174840

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  24. pRL1075

    Plasmid
    #70688
    Purpose
    Use of a vector with a conditionally lethal gene, sacB, to help transfer transposon mutations to a wild type strain
    Depositor
    Peter Wolk
    Article
    Black et al Mol Microbiol. 1993 Jul;9(1):77-84.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70688-plasmid-map-sequence-id-126455

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  29. pRL488

    Plasmid
    #70263
    Purpose
    designed to facilitate transcriptional fusions to luxAB, encoding luciferase, on a plasmid that can replicate in both Escherichia coli and strains of filamentous cyanobacteria.
    Depositor
    Peter Wolk
    Article
    Elhai et al EMBO J. 1990 Oct;9(10):3379-88.
    Type
    Empty backbone
    Use
    Luciferase; Cyanobacteria expression
    Tags
    luxAB
    Expression
    Mutation
    Promoter
    none
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    70263-plasmid-map-sequence-id-130206

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  36. pRL542

    Plasmid
    #70282
    Purpose
    ColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genes
    Depositor
    Peter Wolk
    Article
    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70282-plasmid-map-sequence-id-125718

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Showing: 1 - 50 of 72 results