Skip to main content
Addgene

Acoustic biosensors for ultrasound imaging of enzyme activity.

Lakshmanan A, Jin Z, Nety SP, Sawyer DP, Lee-Gosselin A, Malounda D, Swift MB, Maresca D, Shapiro MG
Nat Chem Biol. 2020 Sep;16(9):988-996. doi: 10.1038/s41589-020-0591-0. Epub 2020 Jul 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
153294WT N-His Ana GvpC in pET28aExpresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli
153295C-His-GvpC-CalpainExpresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli
153296C-His-GvpC-TEVExpresses TEV-sensitive variant of Anabaena flos-aquae GvpC in E.coli
153297N-His-GvpC-ssrAExpresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli
153298pTetR-BCD2-Ana GvpCExpresses wild-type gas vesicle protein C (GvpC) from Anabaena flos-aquae
153299pET28a-T5-ASG_ClpXPExpresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917
153301araBAD-BCD20-ClpP-BCD17-ClpXExpresses clpX and clpP in E.Coli
153302pBEST_OR2-OR1-Pr-UTR1_ClpP-T500Expresses ClpP protease in the cell-free TX-TL system

Antibodies from Article