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Addgene

C-His-GvpC-Calpain
(Plasmid #153295)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153295 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5206
  • Total vector size (bp) 5833
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Calpain-sensitive Gas vesicle protein C (his-tagged)
  • Alt name
    GvpC
  • Species
    Anabaena flos-aquae
  • Insert Size (bp)
    627
  • Mutation
    1) Contains an extra glycine after the methionine at the N-terminus 2) 33 bp calpain recognition and cleavage site flanked by GSGSG linkers, 3) C-terminal His-tag linked to the GvpC sequence with a 3 amino acid (SLE) linker
  • Promoter T7
  • Tag / Fusion Protein
    • His-tag (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C-His-GvpC-Calpain was a gift from Mikhail Shapiro (Addgene plasmid # 153295 ; http://n2t.net/addgene:153295 ; RRID:Addgene_153295)
  • For your References section:

    Acoustic biosensors for ultrasound imaging of enzyme activity. Lakshmanan A, Jin Z, Nety SP, Sawyer DP, Lee-Gosselin A, Malounda D, Swift MB, Maresca D, Shapiro MG. Nat Chem Biol. 2020 Sep;16(9):988-996. doi: 10.1038/s41589-020-0591-0. Epub 2020 Jul 13. 10.1038/s41589-020-0591-0 PubMed 32661379