C-His-GvpC-Calpain
(Plasmid
#153295)
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PurposeExpresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 153295 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5206
- Total vector size (bp) 5833
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCalpain-sensitive Gas vesicle protein C (his-tagged)
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Alt nameGvpC
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SpeciesAnabaena flos-aquae
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Insert Size (bp)627
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Mutation1) Contains an extra glycine after the methionine at the N-terminus 2) 33 bp calpain recognition and cleavage site flanked by GSGSG linkers, 3) C-terminal His-tag linked to the GvpC sequence with a 3 amino acid (SLE) linker
- Promoter T7
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Tag
/ Fusion Protein
- His-tag (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7 forward
- 3′ sequencing primer T7 terminator reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
C-His-GvpC-Calpain was a gift from Mikhail Shapiro (Addgene plasmid # 153295 ; http://n2t.net/addgene:153295 ; RRID:Addgene_153295) -
For your References section:
Acoustic biosensors for ultrasound imaging of enzyme activity. Lakshmanan A, Jin Z, Nety SP, Sawyer DP, Lee-Gosselin A, Malounda D, Swift MB, Maresca D, Shapiro MG. Nat Chem Biol. 2020 Sep;16(9):988-996. doi: 10.1038/s41589-020-0591-0. Epub 2020 Jul 13. 10.1038/s41589-020-0591-0 PubMed 32661379