WT C-His Ana GvpC in pET28a
(Plasmid
#85732)
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PurposeExpresses His-tagged GvpC from Anabaena flos-aquae in E.coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 85732 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5206
- Total vector size (bp) 5818
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGas vesicle protein C (his-tagged)
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Alt nameGvpC
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SpeciesAnabaena flos-aquae
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Insert Size (bp)612
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MutationAdded an extra glycine after the methionine at the N-terminus and have a 3 amino acid linker between GvpC and His-tag
- Promoter T7
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Tag
/ Fusion Protein
- His-tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 forward
- 3′ sequencing primer T7 terminator reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
WT C-His Ana GvpC in pET28a was a gift from Mikhail Shapiro (Addgene plasmid # 85732 ; http://n2t.net/addgene:85732 ; RRID:Addgene_85732) -
For your References section:
Molecular Engineering of Acoustic Protein Nanostructures. Lakshmanan A, Farhadi A, Nety SP, Lee-Gosselin A, Bourdeau RW, Maresca D, Shapiro MG. ACS Nano. 2016 Aug 23;10(8):7314-22. doi: 10.1021/acsnano.6b03364. Epub 2016 Jun 30. 10.1021/acsnano.6b03364 PubMed 27351374