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Addgene

pRL1383a
(Plasmid #70692)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70692 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    RSF1010
  • Vector type
    Bacterial shuttle vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Spectinomycin and Streptomycin for both E.coli and Cyanobacteria
  • Copy number
    Unknown

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MCS: (PT7→)HindIII-SphI-PstI(SbfI)-SalI(HincII)-KpnI-SacI-EcoRI-ClaI-ApaI-SacII-NsiI-NdeI-BglII-AflII-StuI-AvrII-XbaI-AatII-BsiWI-XhoI(PspXI)-SrfI-AscI-RsrII-BamHI(←PSP6)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRL1383a was a gift from Peter Wolk (Addgene plasmid # 70692 ; http://n2t.net/addgene:70692 ; RRID:Addgene_70692)
  • For your References section:

    Paired cloning vectors for complementation of mutations in the cyanobacterium Anabaena sp. strain PCC 7120. Wolk CP, Fan Q, Zhou R, Huang G, Lechno-Yossef S, Kuritz T, Wojciuch E. Arch Microbiol. 2007 Dec;188(6):551-63. Epub 2007 Jul 17. 10.1007/s00203-007-0276-z PubMed 17639350