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Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120.

Elhai J, Vepritskiy A, Muro-Pastor AM, Flores E, Wolk CP
J Bacteriol. 1997 Mar;179(6):1998-2005. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
58494pRL623helper plasmid for bacterial conjugal DNA transfer
70261pRL443Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.
70282pRL542ColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genes
70690pRL1124provides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.

Antibodies from Article