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Addgene

pRL1124
(Plasmid #70690)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70690 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pACYC177
  • Backbone manufacturer
    ATCC
  • Vector type
    Bacterial shuttle vector
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha MCR
  • Growth instructions
    The plasmid must be in appropriately mcr mrr strains such as NEB 10-beta or DH5alpha MCR (or, for the methylases in pRL1124, HB101 will suffice) .
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    avaiM

Gene/Insert 2

  • Gene/Insert name
    eco47iiM

Gene/Insert 3

  • Gene/Insert name
    ecot22iM

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We received pACYC177 from the laboratory of Barry Chelm.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRL1124 was a gift from Peter Wolk (Addgene plasmid # 70690 ; http://n2t.net/addgene:70690 ; RRID:Addgene_70690)
  • For your References section:

    Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120. Elhai J, Vepritskiy A, Muro-Pastor AM, Flores E, Wolk CP. J Bacteriol. 1997 Mar;179(6):1998-2005. PubMed 9068647