pRL1124
(Plasmid
#70690)
-
Purposeprovides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 70690 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepACYC177
-
Backbone manufacturerATCC
-
Vector typeBacterial shuttle vector
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha MCR
-
Growth instructionsThe plasmid must be in appropriately mcr mrr strains such as NEB 10-beta or DH5alpha MCR (or, for the methylases in pRL1124, HB101 will suffice) .
-
Copy numberHigh Copy
Gene/Insert 1
-
Gene/Insert nameavaiM
Gene/Insert 2
-
Gene/Insert nameeco47iiM
Gene/Insert 3
-
Gene/Insert nameecot22iM
Resource Information
-
A portion of this plasmid was derived from a plasmid made byWe received pACYC177 from the laboratory of Barry Chelm.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pRL1124 was a gift from Peter Wolk (Addgene plasmid # 70690 ; http://n2t.net/addgene:70690 ; RRID:Addgene_70690) -
For your References section:
Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120. Elhai J, Vepritskiy A, Muro-Pastor AM, Flores E, Wolk CP. J Bacteriol. 1997 Mar;179(6):1998-2005. PubMed 9068647