pRL648
(Plasmid #70283)

• Purpose: (Empty Backbone) pUC plasmid with inverted polylinker flanking PpsbA-Km marker
• Depositing Lab: Peter Wolk
• Publication: Black et al J Bacteriol. 1994 Apr;176(8):2282-92.

Backbone

• Vector backbone: pUC
• Backbone size (bp): 2686
• Vector type: Bacterial shuttle vector
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alphaMCR
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13-F20
• 3′ sequencing primer: M13-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: From pAH484 (see pRL439, Addgene plasmid 70258) and from transposon Tn5, connected with a 6-bp segment from pUC8, and all set between a pair of BamHI and SmaI sites in symmetric polylinker L.HEHl from Elhai and Wolk, 1988, PMID 2851487.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Additional references:
Elhai J, Wolk CP (1988) A versatile class of positive selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68: 119-138
Kuritz T, Ernst A, Black TA, Wolk CP (1993) High-resolution mapping of genetic loci of Anabaena PCC 7120 required for photosynthesis and nitrogen fixation. Mol Microbiol 8: 101-110