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Plasmid#191182PurposeExpression of EACTB sensor in mammalian cellsDepositorInsertmCherry:ACTB.s1:EGFP-3xMS2
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-CBE2
Plasmid#195368PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTENDepositorInserthA3A/Y130F-XTEN-dLbCas12a-D156R-UGI
UseCRISPR; Gateway compatible ha3a/y130f-xten-dlbcas…TagsExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTC150
Plasmid#70014Purpose'Donor only' control plasmid for gene targeting of tomato ANT1 gene. Contains donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral RepliconDepositorInsertDonor + GVR
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWZL Hygro-NMYC TRF2 deltaB deltaM
Plasmid#16355DepositorInserthTRF2 (TERF2 Human)
UseRetroviralTagsMycExpressionMammalianMutationDeleted Basic Domain (aa1-44) and Myb Domain (aa4…PromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOPO717
Plasmid#195302PurposepBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.DepositorInsertMcCAST Cascade and lacZ spacer 5 in native array
UseTagsExpressionBacterialMutationPromoterArabinose inducible arapBADAvailable sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pBsacA
Plasmid#158650PurposeConstitutive transcription of sacA crRNADepositorInsertsacA crRNA
UseCRISPR and Synthetic Biology; Shuttle vector baci…TagsExpressionBacterialMutationPromoterPvegAvailable sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWZL Hygro-NFLAG TRF1
Plasmid#16277DepositorInserthTRF1 (TERF1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationaa2-439PromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
CeNL-Clathrin_pEGFP
Plasmid#89540PurposeCyan color variant of bright luminescent protein, fused to clathrin light chainDepositorInsertCeNL
UseTagsClathrin light chainExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
6ASB (FBXL19)
Plasmid#119954PurposeBacterial expression for structure determinationDepositorInsertFBXL19 (FBXL19 Human)
UseTags6xHis-TEVExpressionBacterialMutationcontains amino acids 31-153PromoterT7Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
5W9Q (MBD1)
Plasmid#101265PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMBD1 (MBD1 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC22A17_STOP
Plasmid#161201PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC22A17 (SLC22A17 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
5xHRE-MinTK-CRE
Plasmid#141145Purposelentiviral expression vector to generate a hypoxia fate-mapping systemDepositorInsert5xHRE-MinTK-CRE
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
70_pAAV-ProA23-CatCh-GFP-WPRE
Plasmid#125906PurposeTargeting expression to neuronal and glial cell types in mice, non-human primates, and human retinaDepositorInsertCatCh-GFP
UseAAVTagsExpressionMutationPromoterProA23Available sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
4Z3C (TET3)
Plasmid#119969PurposeBacterial expression for structure determinationDepositorInsertTET3 (TET3 Human)
UseTags6xHis-TEVExpressionBacterialMutationcontains amino acids 48-98PromoterT7Available sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NES-mut
Plasmid#42046DepositorInsertTNRC6A (TNRC6A Human)
UseTagsFLAG, HA, and SBPExpressionMammalianMutationF951A, F955A, I958A, F960APromoterAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
BSP188
Plasmid#110917PurposeFor cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator clonedDepositorInsertunc54 Terminator
UseSynthetic Biology; Yeast homologous recombination…TagsExpressionWormMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NLS-mut
Plasmid#42045DepositorInsertTNRC6A (TNRC6A Human)
UseTagsFLAG, HA, and SBPExpressionMammalianMutationR930A, R931A, R932A, R934APromoterAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAM5082
Plasmid#85121PurposePkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization expDepositorInsertPkaiB-KaiC-ECFP
UseTagsECFPExpressionBacterialMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWZL Hygro-NFLAG TRF1 deltaA
Plasmid#16278DepositorInserthTRF1 (TERF1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationDeleted acidic domain (aa1-66)PromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only