160,048 results

1. pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro

   Plasmid

   #73037

   Purpose

   Express Nluc and copGFP dual reporter

   Depositor

   Kazuhiro Oka

   Insert

   Nluc-P2A-copGFP-T2A-Puro
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF_PE2

   Plasmid

   #199267

   Purpose

   Mammalian expression of SpCas9 prime editor 2 under EF1α promoter

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   PE2-P2A-mp53DD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Δ40-903

   Promoter

   Available since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5_mCherry_P2AT2A_GFP-nb-KS-FtoA

   Plasmid

   #238234

   Purpose

   For overexpression of mCherry_P2AT2A_GFP-nb-KS-FtoA

   Depositor

   Denes Hnisz

   Insert

   mCherry_P2AT2A_GFP-nb-KS-FtoA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

   Plasmid

   #198468

   Purpose

   E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

   Depositor

   Viviana Risca

   Article

   Soroczynski et al bioRxiv 2024.07.11.602973

   Insert

   10His-pG-Tn5 (E54K, L372P)
   

   Use

   Tags

   10His-pG

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   Consensus φ10 T7 Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCXLE-SOX2-17-p2a-KLF4

   Plasmid

   #193290

   Purpose

   episomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversion

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   SOX2-17-p2a-KLF4 (SOX2, KLF4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SOX2-17 is engineered highly cooperative chimeric…

   Promoter

   CAG

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

   Plasmid

   #175573

   Purpose

   Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiFUCCI(CA)5

   Plasmid

   #223176

   Purpose

   Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

   Depositor

   Neville Sanjana

   Article

   Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

   Insert

   FUCCI(CA)5
   

   Use

   Lentiviral

   Tags

   AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. plenti.CMV.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

   Plasmid

   #236407

   Purpose

   Expresses cytosolic low affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEE083

   Plasmid

   #149279

   Purpose

   T-DNA encoding TRV2 complete genome with MCS

   Depositor

   Daniel Voytas

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-C1-AR Q48

    Plasmid

    #86428

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Poly-glutamine expansion, G130D

    Promoter

    CMV

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP VAMP3

    Plasmid

    #42310

    Depositor

    Thierry Galli

    Article

    Galli et al Mol Biol Cell. 1998 Jun;9(6):1437-48.

    Insert

    VAMP 3 (VAMP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. murine TCR OTII-2A.pMIG II

    Plasmid

    #52112

    Purpose

    retroviral expression of both TCRalpha and TCRbeta using a single vector

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Inserts

    T cell receptor alpha chain (Tcra Mouse)
    T cell receptor beta chain (Tcrb Mouse)
    

    Use

    Retroviral

    Tags

    P2A (from porcine teschovirus-1)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRFHUE-eGFP

    Plasmid

    #89469

    Purpose

    GFP labelling of filamentous fungi by Agrobacterium tumefaciens mediated transformation

    Depositor

    Luis González-Candelas

    Article

    Crespo-Sempere et al Int J Food Microbiol. 2011 Aug 2;148(2):

    Insert

    eGFP
    

    Use

    Binary plasmid for atmt of filamentous fungi

    Tags

    Expression

    Mutation

    Promoter

    A. nidulans gpdA

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-PTEN

    Plasmid

    #13039

    Depositor

    Alonzo Ross

    Article

    Liu et al J Cell Biochem. 2005 Oct 1. 96(2):221-34

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmal_c5x_RNAse_Inhib

    Plasmid

    #153314

    Purpose

    Bacterial expression of an RNase inhibitor

    Depositor

    Philippa Marrack, Drew Endy

    Insert

    RNAse Inhibitor
    

    Use

    Tags

    Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. VPS13C^mClover3

    Plasmid

    #118760

    Purpose

    VPS13C internally tagged with mClover3 at residue 1914.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13C (VPS13C Human)
    

    Use

    Tags

    mClover3 inserted at position aa 1914

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-GFP/Cre

    Plasmid

    #49056

    Purpose

    Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.

    Depositor

    Fred Gage

    Article

    Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

    Insert

    Cre
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    EGFP, Myc, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. HaloTag-bActinCDS-bActinUTR-MS2V5

    Plasmid

    #102718

    Purpose

    Halo-Actin reporter for detection of both mRNA and protein

    Depositor

    Robert Singer

    Article

    Yoon et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    HaloTag-bActinCDS-bActin3'UTR-MS2V5
    

    Use

    Lentiviral

    Tags

    HaloTag and MS2V5

    Expression

    Mutation

    Promoter

    UbC

    Available since

    Nov. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmEmerald-ERGIC-53

    Plasmid

    #170717

    Purpose

    Expresses mEmerald-tagged human ERGIC-53 at high level in mammalian cells.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    ERGIC-53 (LMAN1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CMV-SauriCas9

    Plasmid

    #135964

    Purpose

    Expresses SauriCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge