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Addgene

pEGFP-C1-AR Q48
(Plasmid #86428)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86428 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech (Takara)
  • Backbone size w/o insert (bp) 4731
  • Total vector size (bp) 8026
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Growth instructions
    New plasmid preps must be sequenced to verify (there can be loss or addition of poly-glutamine repeats).
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Androgen receptor (AR)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2850
  • Mutation
    Poly-glutamine expansion, G130D
  • GenBank ID
    NM_000044.4
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xma I (not destroyed)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's sequencing results found that this plasmid carries an extra Q in the poly-glutamine expansion bring the total to 49 rather than 48 and found a G130D mutation compared to NCBI reference sequences. These mutations are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1-AR Q48 was a gift from Michael Mancini (Addgene plasmid # 86428 ; http://n2t.net/addgene:86428 ; RRID:Addgene_86428)
  • For your References section:

    Polyglutamine-expanded androgen receptors form aggregates that sequester heat shock proteins, proteasome components and SRC-1, and are suppressed by the HDJ-2 chaperone. Stenoien DL, Cummings CJ, Adams HP, Mancini MG, Patel K, DeMartino GN, Marcelli M, Weigel NL, Mancini MA. Hum Mol Genet. 1999 May . 8(5):731-41. 10.1093/hmg/8.5.731 PubMed 10196362