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PurposeFluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 86428 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech (Takara)
- Backbone size w/o insert (bp) 4731
- Total vector size (bp) 8026
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Modifications to backbonenone
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Growth instructionsNew plasmid preps must be sequenced to verify (there can be loss or addition of poly-glutamine repeats).
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAndrogen receptor (AR)
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SpeciesH. sapiens (human)
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Insert Size (bp)2850
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MutationPoly-glutamine expansion, G130D
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GenBank IDNM_000044.4
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Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xma I (not destroyed)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene's sequencing results found that this plasmid carries an extra Q in the poly-glutamine expansion bring the total to 49 rather than 48 and found a G130D mutation compared to NCBI reference sequences. These mutations are not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-C1-AR Q48 was a gift from Michael Mancini (Addgene plasmid # 86428 ; http://n2t.net/addgene:86428 ; RRID:Addgene_86428) -
For your References section:
Polyglutamine-expanded androgen receptors form aggregates that sequester heat shock proteins, proteasome components and SRC-1, and are suppressed by the HDJ-2 chaperone. Stenoien DL, Cummings CJ, Adams HP, Mancini MG, Patel K, DeMartino GN, Marcelli M, Weigel NL, Mancini MA. Hum Mol Genet. 1999 May . 8(5):731-41. 10.1093/hmg/8.5.731 PubMed 10196362