160,076 results

1. pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry

   Plasmid

   #193465

   Purpose

   Dn29 Donor with promoter for mcherry and puro expression

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Dn29_attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKK-TEV-CyOFP1

   Plasmid

   #105799

   Purpose

   Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-CyOFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-tag

   Plasmid

   #55180

   Purpose

   FLAG-tag

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   FLAG
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTriEx-mCherry-PA-Rac1-T17N

   Plasmid

   #22029

   Purpose

   Expression of photoactivatable Rac1 (dominant-negative mutant).

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
   

   Use

   Tags

   6xHis and mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…

   Promoter

   CMV, p10

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. σ1-mCherry

   Plasmid

   #186578

   Purpose

   Expression of adaptor protein 1 (AP1) sigma subunit AP1S1 tagged with mCherry

   Depositor

   Stephen Royle

   Article

   Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

   Insert

   AP1S1 (AP1S1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRIP-CMV-Puro-2A

   Plasmid

   #102611

   Purpose

   Control lentivector expressing Puro resistance and gene of interest from CMV promoter

   Depositor

   Nicolas Manel

   Article

   Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. XE251 pCDNA3.1(zeo)-hDsh3

   Plasmid

   #16758

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   FLAG-hDsh3 (DVL3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-pcDNA3-PKCgamma-cys1Acys1B

   Plasmid

   #21190

   Depositor

   Tobias Meyer

   Article

   Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

   Insert

   PKCgamma-cys1Acys1B (Prkcg Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamm…

   Promoter

   Available since

   July 15, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. Jag1-HA pIRES

   Plasmid

   #17336

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Koutelou et al J Biol Chem. 2007 Dec 12. ():.

   Insert

   Jagged1 (Jag1 Rat)
   

   Use

   Tags

   HA X3

   Expression

   Mammalian

   Mutation

   A silent C->G mutation at 3237

   Promoter

   Available since

   April 24, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAV1-K*R-mEGFP

    Plasmid

    #27766

    Depositor

    Ari Helenius

    Article

    Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

    Insert

    caveolin-1, lysine null mutant (CAV1 Human)
    

    Use

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    All lysines mutated to arginines (K*R)

    Promoter

    Available since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCerulean-VSVG

    Plasmid

    #11913

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

    Insert

    VSVG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…

    Promoter

    Available since

    Sept. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 1013 pcDNA3 T7 Akt1 T308A S473A

    Plasmid

    #9030

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    Akt1 T308A S473A (AKT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T308A S473A

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. sfGFP-Caveolin-C-10

    Plasmid

    #56366

    Purpose

    Localization: Membrane, Excitation: 485, Emission: 507

    Depositor

    Michael Davidson

    Insert

    Caveolin (CAV1 Human)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pcDNA-SET-FLAG-HA

    Plasmid

    #24998

    Depositor

    Judy Lieberman

    Article

    Yan et al PLoS Pathog. 2009 Mar . 5(3):e1000327.

    Insert

    SET (SET Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-hSyn Coff/FonEYFP

    Plasmid

    #55652

    Purpose

    Cre-off/Flp-on EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    eYFP with introns
    

    Use

    AAV; Cre off/flp on eyfp

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-DIO-EF1α-mKate2-PDE4D3-Cat

    Plasmid

    #169128

    Purpose

    AAV plasmid containing a mKate2 and a truncated PDE4D3

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Insert

    mKate2-PDE4DCatL
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    truncated, Catalatic domain only

    Promoter

    EF1a

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pShuttle-Cre-HA

    Plasmid

    #16583

    Depositor

    Bert Vogelstein

    Article

    Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

    Insert

    cre recombinase (cre P1 bacteriophage)
    

    Use

    Adenoviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCW57-mcGAS

    Plasmid

    #185458

    Purpose

    Inducible FLAG-tagged mouse cGAS

    Depositor

    Shitao Li

    Article

    Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

    Insert

    cGAS
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mEmerald-Claudin7-C-12

    Plasmid

    #54041

    Purpose

    Localization: Tight Junctions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Claudin7 (CLDN7 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pD40-His/V5-c-Myc

    Plasmid

    #45597

    Depositor

    Rosalie Sears

    Article

    Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

    Insert

    c-Myc (Myc Mouse)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge