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PurposeGreen fluorescent probe for cGMP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 86867 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1 (-)
- Backbone size w/o insert (bp) 5358
- Total vector size (bp) 6494
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGreen cGull
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Insert Size (bp)1135
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CMV-Forward, 5'-CGC AAA TGG GCG GTA GGC GTG-3' (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Green cGull was a gift from Tetsuya Kitaguchi (Addgene plasmid # 86867 ; http://n2t.net/addgene:86867 ; RRID:Addgene_86867) -
For your References section:
Generation of a cGMP Indicator with an Expanded Dynamic Range by Optimization of Amino Acid Linkers between a Fluorescent Protein and PDE5alpha. Matsuda S, Harada K, Ito M, Takizawa M, Wongso D, Tsuboi T, Kitaguchi T. ACS Sens. 2017 Jan 27;2(1):46-51. doi: 10.1021/acssensors.6b00582. Epub 2016 Dec 29. 10.1021/acssensors.6b00582 PubMed 28722423