Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.
Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
Nature. 2014 Dec 10. doi: 10.1038/nature14136.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 61422 | dCAS9-VP64_GFP | Expresses dCAS9-VP64 activator with 2A GFP |
| 61423 | MS2-P65-HSF1_GFP | Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector. |
| 61424 | sgRNA(MS2) cloning backbone | sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences. |
| 61425 | lenti dCAS-VP64_Blast | 3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE) |
| 61426 | lenti MS2-P65-HSF1_Hygro | lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2 |
| 61427 | lenti sgRNA(MS2)_zeo backbone | 3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences. |
| 73795 | lenti sgRNA(MS2)_puro backbone | 3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences. |