Skip to main content
Addgene
Showing: 11201 - 11250 of 28579 results

  1. pAAV-EF1a-N-CretrcintG

    Plasmid
    #69570
    Purpose
    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells
    Depositor
    Connie Cepko
    Article
    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do
    Has Service
    AAV1
    Insert
    kozak consensus - N-CretrcintG
    Use
    AAV and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69570-plasmid-map-sequence-id-127742

    Enlarge

  2. pDC-UAS-nBitbow1.0(miniW)

    Plasmid
    #138329
    Purpose
    Drosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.
    Depositor
    Dawen Cai
    Article
    Veling et al Cell Rep. 2019 Dec 3;29(10):3303-3312.e3
    Inserts
    mAmetrine
    tdKatushka2
    mNeonGreen
    mTFP1
    mKusabira-Orange2
    Use
    Tags
    human H2B
    Expression
    Insect
    Mutation
    Promoter
    10xUAS
    Available since
    March 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    138329-plasmid-map-sequence-id-275089

    Enlarge

  3. pAAV-EF1a-C-CreintG

    Plasmid
    #69571
    Purpose
    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells
    Depositor
    Connie Cepko
    Article
    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do
    Has Service
    AAV1
    Insert
    kozak consensus - C-CreintG
    Use
    AAV and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69571-plasmid-map-sequence-id-127747

    Enlarge

  10. pAAV.Syn.Flex.GCaMP6f.WPRE.SV40

    Plasmid
    #100833
    Purpose
    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter
    Depositor
    GENIE Project, Douglas Kim
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G
    Promoter
    Synapsin
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    100833-plasmid-map-sequence-id-198271

    Enlarge

  21. pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid
    #75154
    Purpose
    Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    H2B-mCerulean3-P2A-mKOFP2-CAAX
    Use
    Middle entry vector for multisite gateway three f…
    Tags
    Expression
    Mutation
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75154-plasmid-map-sequence-id-142108

    Enlarge

  38. scFLARE1-p2A-eGFP

    Plasmid
    #158699
    Purpose
    Mammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection marker
    Depositor
    Alice Ting
    Article
    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p
    Insert
    scFLARE1-p2A-eGFP
    Use
    AAV
    Tags
    Flag, V5. eGFP
    Expression
    Mammalian
    Mutation
    Truncated form of TEV protease (220-242 amino aci…
    Promoter
    CMV
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158699-plasmid-map-sequence-id-315073

    Enlarge

  42. pAAV.Syn.Flex.GCaMP6s.WPRE.SV40

    Plasmid
    #100845
    Purpose
    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter
    Depositor
    GENIE Project, Douglas Kim
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G
    Promoter
    Synapsin
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    100845-plasmid-map-sequence-id-241433

    Enlarge

  44. HSC1-HS4-GiP

    Plasmid
    #58540
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Core HS4 insulator sequence (dimer)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1-alpha
    Available since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58540-plasmid-map-sequence-id-90629

    Enlarge

  50. pCAG-lox-rox-STOP-rox-lynScarlet-lox-Lyn-NeonGreen

    Plasmid
    #175259
    Purpose
    dual plasmid labelling membrane target protein of different neuronal population with different flurophore
    Depositor
    Frank Bradke, Sebastián Dupraz
    Article
    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37
    Insert
    Lox-rox-STOP-rox-lyn-mScarlet-pA-lox
    Use
    Cre/Lox; Dre/rox
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    None (wt)
    Promoter
    pCAG
    Available since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    175259-plasmid-map-sequence-id-375583

    Enlarge

Showing: 11201 - 11250 of 28579 results