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Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease

Plasmid Kit

#1000000087

Purpose

Tol2 Zebrafish toolkit: 4 promoters, 6 fluorophores, MultiSite Gateway (Invitrogen) compatible and cloning sites. Production transgenic zebrafish for neurodegenerative disorders

Depositor

Nicholas Cole

Available since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only
PB_iNEUROD1_P2A_GFP_Puro

Plasmid

#168803

Purpose

PiggyBac transposon construct with doxycycline inducible NEUROD1 + GFP expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

NEUROD1 (NEUROD1 Synthetic, Human)

Use

Synthetic Biology; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pTet-O-NEUROD1-T2A-PuroR

Plasmid

#162338

Purpose

Lentiviral expression of NEUROD1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

NEUROD1 (NEUROD1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTet-O-NEUROG1-T2A-PuroR

Plasmid

#162339

Purpose

Lentiviral expression of NEUROG1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

NEUROG1 (NEUROG1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTet-O-NEUROG3-T2A-PuroR

Plasmid

#162341

Purpose

Lentiviral expression of NEUROG3 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

NEUROG3 (NEUROG3 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Mutation

Promoter

Tet-ON

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLV[TetOn]-Puro-TRE3G>mNeurog2

Plasmid

#198754

Purpose

Vector encodes the gene for murine neurogenin-2 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

Depositor

Nael Nadif Kasri

Article

Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

Insert

Neurogenin-2 (Neurog2 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE3G

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-Neuromodulin-IRES-Puromycin

Plasmid

#134666

Purpose

Expresses EGFP-tagged plasma membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Neuromodulin (GAP43 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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LentiU6-hNEUROD1 gRNA_1-MS2-Puro

Plasmid

#192676

Purpose

Lentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human NEUROD1 activating gRNA #1 (NEUROD1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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LentiU6-mNeurog2 gRNA_1-MS2-Puro

Plasmid

#192680

Purpose

Lentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Mouse Neurog2 activating gRNA #1 (Neurog2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-FLAG-Neuroserpin oloop

Plasmid

#58262

Purpose

Retroviral vector for expressing mutated Neuroserpin in mammalian cells

Depositor

Joan Massague

Article

Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

Insert

Neuroserpin (SERPINI1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Mutant of neuroserpin lacking five residues from …

Promoter

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

Plasmid

#40731

Depositor

Daniel Gerlich

Article

Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

Insert

Aurora B FRET-sensor

Use

Tags

CyPet and YPet

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

Plasmid

#40571

Depositor

Daniel Gerlich

Article

Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

Insert

Aurora B FRET-sensor N-terminally fused to histone 2B

Use

Tags

CyPet and YPet

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

Plasmid

#121174

Purpose

Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines

Depositor

Timo Otonkoski

Insert

MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro

Plasmid

#89994

Purpose

Donor template for generation of NGN3-ntdTomato reporter cell lines

Depositor

Timo Otonkoski

Insert

NGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

none

Available since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

Plasmid

#121175

Purpose

Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines

Depositor

Timo Otonkoski

Insert

GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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Donor_ANKRD1_KO_Puro

Plasmid

#186667

Purpose

Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO Puromycin (ANKRD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_YTHDF2_KO_Puro

Plasmid

#186670

Purpose

Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Puromycin (YTHDF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EGFP_Donor

Plasmid

#178088

Purpose

HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_Puro_sgTP53

Plasmid

#187819

Purpose

pLentiCRISPR that is selectable with puromycin with an sgRNA targeting TP53

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgTP53

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.DEST.puro

Plasmid

#32682

Purpose

Lentiviral cDNA/shRNA gateway destination vectors

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2014

Availability

Academic Institutions and Nonprofits only

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