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Ultrasensitive fluorescent proteins for imaging neuronal activity.

Chen TW, Wardill TJ, Sun Y, Pulver SR, Renninger SL, Baohan A, Schreiter ER, Kerr RA, Orger MB, Jayaraman V, Looger LL, Svoboda K, Kim DS
Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
40753pGP-CMV-GCaMP6sMammalian expression of ultrasensitive protein calcium sensor
40754pGP-CMV-GCaMP6mMammalian expression of ultrasensitive protein calcium sensor
40755pGP-CMV-GCaMP6fMammalian expression of ultrasensitive protein calcium sensor
100833pAAV.Syn.Flex.GCaMP6f.WPRE.SV40AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter
100835pAAV.CAG.Flex.GCaMP6f.WPRE.SV40AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter
100836pAAV.CAG.GCaMP6f.WPRE.SV40AAV expression of ultrasensitive protein calcium sensor from CAG promoter
100837pAAV.Syn.GCaMP6f.WPRE.SV40AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter
100838pAAV.Syn.Flex.GCaMP6m.WPRE.SV40AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter
100839pAAV.CAG.Flex.GCaMP6m.WPRE.SV40AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter
100840pAAV.CAG.GCaMP6m.WPRE.SV40AAV expression of ultrasensitive protein calcium sensor from CAG promoter
100841pAAV.Syn.GCaMP6m.WPRE.SV40AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter
100842pAAV.CAG.Flex.GCaMP6s.WPRE.SV40AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter
100843pAAV.Syn.GCaMP6s.WPRE.SV40AAV expression of ultrasensitive protein calcium sensor from Synapsin promoter
100844pAAV.CAG.GCaMP6s.WPRE.SV40AAV expression of ultrasensitive protein calcium sensor from CAG promoter
100845pAAV.Syn.Flex.GCaMP6s.WPRE.SV40AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoter

Antibodies from Article