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In vivo measurement of afferent activity with axon-specific calcium imaging.

Broussard GJ, Liang Y, Fridman M, Unger EK, Meng G, Xiao X, Ji N, Petreanu L, Tian L
Nat Neurosci. 2018 Sep;21(9):1272-1280. doi: 10.1038/s41593-018-0211-4. Epub 2018 Aug 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
111261pAAV-hSynapsin1-axon-GCaMP6mFor enriched expression of GCaMP6m in axons
111262pAAV-hSynapsin1-axon-GCaMP6sFor enriched expression of GCaMP6s in axons
112000pAAV-hSynapsin1-axon-GCaMP6fFor enriched expression of GCaMP6f in axons
112004pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2For enriched expression of GCaMP6s in axons with cytosolic expression of mKate2
112005pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3For enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3
112006pAAV-hSynapsin1-GCaMP6s-P2A-mKate2For co-expression of GCaMP6s with mKate2
112007pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3For co-expression of GCaMP6s with mRuby3
112008pAAV-hSynapsin1-FLEx-axon-GCaMP6s-P2A-mRuby3For cre-dependent enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3
112010pAAV-hSynapsin1-FLEx-axon-GCaMP6sFor cre-dependent enriched expression of GCaMP6s in axons

Antibodies from Article