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Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors.

Patriarchi T, Cho JR, Merten K, Howe MW, Marley A, Xiong WH, Folk RW, Broussard GJ, Liang R, Jang MJ, Zhong H, Dombeck D, von Zastrow M, Nimmerjahn A, Gradinaru V, Williams JT, Tian L
Science. 2018 Jun 29;360(6396). pii: science.aat4422. doi: 10.1126/science.aat4422. Epub 2018 May 31. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
111053pCMV-dLight1.1Expresses dLight1.1 in mammalian cells
111054pCMV-dLight1.2Expresses dLight1.2 in mammalian cells
111055pCMV-dLight1.3aExpresses dLight1.3a in mammalian cells
111056pCMV-dLight1.3bExpresses dLight1.3b in mammalian cells
111057pCMV-dLight1.4Expresses dLight1.4 in mammalian cells
111058pCMV-dLight1.5Expresses dLight1.5 in mammalian cells
111061pCMV-nLight3.1Expresses nLight3.1 in mammalian cells
111066pAAV-hSyn-dLight1.1To generate Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter
111067pAAV-CAG-dLight1.1To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter
111068pAAV-hSyn-dLight1.2To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter
111069pAAV-CAG-dLight1.2To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter
125560pAAV-CAG-dLight1.3bFor expression of the genetically encoded dopamine sensor dLight1.3b
135762pAAV-syn-dLight1.3bFor expression of the genetically encoded dopamine sensor dLight1.3b

Antibodies from Article