-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 17544 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBabe-puro
- Backbone size w/o insert (bp) 5200
-
Vector typeMammalian Expression, Retroviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameB-Raf
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2477
-
MutationV600E
-
Entrez GeneBRAF (a.k.a. B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBABE-5
- 3′ sequencing primer pBABE-3 (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made bypcDNA3 B-Raf (WT); provided by P. Stork, Oregon Health Sciences University, Portland, OR
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBabe B-Raf V600E was a gift from Channing Der (Addgene plasmid # 17544 ; http://n2t.net/addgene:17544 ; RRID:Addgene_17544) -
For your References section:
Context-dependent roles of mutant B-Raf signaling in melanoma and colorectal carcinoma cell growth. Hao H, Muniz-Medina VM, Mehta H, Thomas NE, Khazak V, Der CJ, Shields JM. Mol Cancer Ther. 2007 Aug . 6(8):2220-9. 10.1158/1535-7163.MCT-06-0728 PubMed 17699719