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Addgene

pBabe B-Raf V600E
(Plasmid #17544)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17544 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBabe-puro
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    B-Raf
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2477
  • Mutation
    V600E
  • Entrez Gene
    BRAF (a.k.a. B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pcDNA3 B-Raf (WT); provided by P. Stork, Oregon Health Sciences University, Portland, OR
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe B-Raf V600E was a gift from Channing Der (Addgene plasmid # 17544 ; http://n2t.net/addgene:17544 ; RRID:Addgene_17544)
  • For your References section:

    Context-dependent roles of mutant B-Raf signaling in melanoma and colorectal carcinoma cell growth. Hao H, Muniz-Medina VM, Mehta H, Thomas NE, Khazak V, Der CJ, Shields JM. Mol Cancer Ther. 2007 Aug . 6(8):2220-9. 10.1158/1535-7163.MCT-06-0728 PubMed 17699719