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Showing: 3601 - 3650 of 156588 results

  9. pAN7.1

    Plasmid
    #168129
    Purpose
    Expresses hygR from the gdpA promoter with a trpC terminator. Typically used in Aspergillus species as a selection marker for resistance to hygromycin.
    Depositor
    Michael Bromley
    Article
    Zhao et al Curr Protoc Microbiol. 2019 Sep;54(1):e8
    Insert
    HygR gene
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    gdpA
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    168129-plasmid-map-sequence-id-330738

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  16. pCDB179-TurboPETase

    Plasmid
    #218693
    Purpose
    Encodes codon optimized TurboPETase enzyme for bacterial expression
    Depositor
    Gregg Beckham
    Insert
    TurboPETase
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218693-plasmid-map-sequence-id-434224

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  19. pRPA70-IRES-RPA32-IRES-RPA14

    Plasmid
    #211432
    Purpose
    mammalian expression of human RPA70 and RPA32 and RPA14
    Depositor
    Helmut Pospiech
    Insert
    RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211432-plasmid-map-sequence-id-420528

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  27. AICSDP-80:UBTF-HaloTag

    Plasmid
    #133963
    Purpose
    Homology arms and HaloTag-linker sequence for N-terminus tagging of human UBTF
    Depositor
    Allen Institute for Cell Science
    Insert
    UBTF Homology Arms with HaloTag-linker (UBTF Human)
    Use
    CRISPR
    Tags
    HaloTag-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    133963-plasmid-map-sequence-id-264347

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  28. pcDNA3.1-kappa-myc-dL5-2xG4S-TMst

    Plasmid
    #73206
    Purpose
    Expresses myc-dL5(E52D)-TM (PDGFR derived) on the surface of mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    kappa-myc-dL5-2XG4S-TM (MYC Human)
    Use
    Tags
    The FAP is fused with 2 copies of a G4S linker an…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73206-plasmid-map-sequence-id-139721

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  31. pcDNA3-endo-Nb80

    Plasmid
    #205518
    Purpose
    Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    endo-Nb80
    Use
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205518-plasmid-map-sequence-id-409484

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  32. pLVX-FLAG-mCherry-C1-centrin-1

    Plasmid
    #73333
    Purpose
    Lentiviral vector encoding centrin-1 with N-terminal FLAG and mCherry tags
    Depositor
    Manuel Thery
    Insert
    centrin-1 (CETN1 Human)
    Use
    Lentiviral
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    73333-plasmid-map-sequence-id-139042

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  33. pX330 _CRISPIE donor B9

    Plasmid
    #172850
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B9 (Zhong et al, eLife 2021), mEGFP translational phase (2-2), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 2-2) encoding mEGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172850-plasmid-map-sequence-id-341005

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  44. AAV-PR rep/cap

    Plasmid
    #197565
    Purpose
    encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery
    Depositor
    Casey Maguire
    Article
    Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089
    Inserts
    AAV Rep genes
    AAV9 VP1 Cap gene with PR insert
    Use
    AAV
    Tags
    Expression
    Mutation
    contains 7mer insert PRPPSTH between amino acids …
    Promoter
    Available since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    197565-plasmid-map-sequence-id-388046

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  45. pX330 _CRISPIE donor B8

    Plasmid
    #172849
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B8 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 1-1) encoding mEGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172849-plasmid-map-sequence-id-340928

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  47. Split 'n coexpress CUL3-RBX1

    Plasmid
    #52293
    Purpose
    bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1
    Depositor
    Brenda Schulman
    Article
    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1
    Inserts
    Cul3 N-terminal domain (CUL3 Human)
    Cul3 C-terminal domain (CUL3 Human)
    RBX1 (Rbx1 Mouse)
    Use
    Tags
    GST, IRES, and TEV
    Expression
    Bacterial
    Mutation
    contains aa 1-309, contains aa 310-768, and conta…
    Promoter
    IRES and tac
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  49. pBBR15.2-2H12.D11opt-scarRef

    Plasmid
    #221150
    Purpose
    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen2.1
    mScarlet-I
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen2.1)
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221150-plasmid-map-sequence-id-437166

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  50. pWZL Hygro

    Plasmid
    #18750
    Depositor
    Scott Lowe
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 3, 2008
    Availability
    Academic Institutions and Nonprofits only
Showing: 3601 - 3650 of 156588 results