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Addgene
Showing: 3601 - 3650 of 144247 results
  1. pMM178

    Plasmid
    #61270
    Purpose
    Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.
    Depositor
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    pLtetO, native, native (respectively)
    Availability
    Academic Institutions and Nonprofits only
  2. pMM441

    Plasmid
    #61271
    Purpose
    Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.
    Depositor
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pRC319

    Plasmid
    #61272
    Purpose
    Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.
    Depositor
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. NOVA1 eGFP

    Plasmid
    #61275
    Purpose
    GFP-tagged ORF clone of Homo sapiens neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1
    Insert
    NOVA1 (NOVA1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  5. pmIL-6 -231

    Plasmid
    #61287
    Purpose
    drives luciferase via mouse IL-6 promoter, truncated (-231)
    Depositor
    Insert
    Truncated IL-6 promoter
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of 1046 nucleotides from the 5' end…
    Promoter
    IL-6 promoter (truncated)
    Availability
    Academic Institutions and Nonprofits only
  6. pmIL-6 mut C/EBP

    Plasmid
    #61291
    Purpose
    drives luciferase from mouse IL-6 promoter with mutant C/EBP (NF-IL-6) site
    Depositor
    Insert
    IL-6 promoter (Il6 Mouse)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Mutated C/EBP binding site from ACATTGTGCAATCT to…
    Promoter
    IL-6 promoter with mutant C/EBP binding site
    Availability
    Academic Institutions and Nonprofits only
  7. pmIL-6 mut AP-1+C/EBP

    Plasmid
    #61292
    Purpose
    drives luciferase from mouse IL-6 promoter with mutations in both AP-1 & C/EBP sites
    Depositor
    Insert
    IL-6 promoter (Il6 Mouse)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Mutated both AP-1 binding site from TGAGTCT to TG…
    Promoter
    IL-6 promoter with mutant AP-1 and C/EBP binding …
    Availability
    Academic Institutions and Nonprofits only
  8. pmIL-6 mut NF-kB

    Plasmid
    #61293
    Purpose
    drives luciferase from mouse IL-6 promoter with mutation in NF-kB site
    Depositor
    Insert
    IL-6 promoter (Il6 Mouse)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Mutated NF-kB binding site from GTGGGATTTTCCCA to…
    Promoter
    IL-6 promoter with mutant NF-kB binding site
    Availability
    Academic Institutions and Nonprofits only
  9. pEGFP-N3 alpha-SSeCKS

    Plasmid
    #61294
    Purpose
    Mammalian expression of GFP-alpha-SSeCKS
    Depositor
    Insert
    alpha SSeCKS (Akap12 Rat)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  10. pBluescript alpha-SSeCKS

    Plasmid
    #61295
    Purpose
    contains rat alpha SSeCKS cDNA
    Depositor
    Insert
    alpha SSeCKS (Akap12 Rat)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cDNA with 1kb of 3' UTR and 150bp of 5'…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  11. pVYD

    Plasmid
    #61302
    Purpose
    Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to SYFP2
    Depositor
    Type
    Empty backbone
    Use
    Tags
    SYFP2
    Expression
    Bacterial
    Mutation
    Promoter
    PV10
    Availability
    Academic Institutions and Nonprofits only
  12. pVHD

    Plasmid
    #61303
    Purpose
    Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to HA tag
    Depositor
    Type
    Empty backbone
    Use
    Tags
    HA
    Expression
    Bacterial
    Mutation
    Promoter
    PV10
    Availability
    Academic Institutions and Nonprofits only
Showing: 3601 - 3650 of 144247 results