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Purposeencodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197565 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAR9
- Total vector size (bp) 7486
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameAAV Rep genes
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Speciesparvovirus
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Insert Size (bp)1863
Gene/Insert 2
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Gene/Insert nameAAV9 VP1 Cap gene with PR insert
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Speciesparvovirus
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Insert Size (bp)2229
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Mutationcontains 7mer insert PRPPSTH between amino acids 588 and 589 of AAV9 VP1 protein
Resource Information
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Supplemental Documents
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-PR rep/cap was a gift from Casey Maguire (Addgene plasmid # 197565 ; http://n2t.net/addgene:197565 ; RRID:Addgene_197565) -
For your References section:
An engineered AAV capsid mediates efficient transduction of pericytes and smooth muscle cells of the brain vasculature. Ramirez SH, Hale J, McCarthy S, Cardenas CL, Dona K, Hanlon KS, Hudry E, De La Cruz D, Ng C, Das S, Nguyen DM, Nammour J, Bennett RE, Andrews A, Musolino PL, Maguire CA. Hum Gene Ther. 2023 Jun 27. doi: 10.1089/hum.2022.211. 10.1089/hum.2022.211 PubMed 37376759
