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Addgene

pAG426GAL-ccdB-EGFP
(Plasmid #14203)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14203 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Cloning Grade DNA 14203-DNA.cg 2 µg of cloning grade DNA in Tris buffer 1 $105

Backbone

  • Vector backbone
    pRS426
  • Vector type
    Yeast Expression ; Gateway Destination
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    DB3.1, ccdB Survival
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Type of plasmid: 2-micron.

This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com

Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.

Information for Cloning Grade DNA (Catalog # 14203-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $105 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG426GAL-ccdB-EGFP was a gift from Susan Lindquist (Addgene plasmid # 14203 ; http://n2t.net/addgene:14203 ; RRID:Addgene_14203)
  • For your References section:

    A suite of Gateway cloning vectors for high-throughput genetic analysis in Saccharomyces cerevisiae. Alberti S, Gitler AD, Lindquist S. Yeast. 2007 Oct . 24(10):913-9. 10.1002/yea.1502 PubMed 17583893