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Plasmid#164506PurposeTo generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES(Myb)-T7-Halo-PDGFR_TM-T2A-Puro.DepositorTypeEmpty backboneUseBxbi site specific integrationTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only
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StarShip03
Plasmid#164507PurposeTo generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES2-T7-Halo-PDGFR_TM-T2A-Puro.DepositorTypeEmpty backboneUseBxbi site specific integrationTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
SHIP2-ΔPS
Plasmid#214901Purposeexpression of the truncated variants of the SHIP2 without phosphatase domainDepositorInserthuman SHIP2 delta aa 419-739 (INPPL1 Human)
UseTagsV5/HisExpressionMammalianMutationdelta 419-739aaPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cre Shine
Plasmid#37404PurposeThis is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.DepositorInsertCre Shine
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
Human SHIP2
Plasmid#124645PurposeExpresses HIS tagged human SHIP2 in COS-7/HEK cellsDepositorInsertINPPL1 (INPPL1 Human)
UseTagsHis Xpress (N-terminal)ExpressionMammalianMutationcoding sequence starts at amino acid 18 as calcul…PromoterAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shIFNAR
Plasmid#127699PurposeDoxycyclin inducible shRNA knockdown of mouse IFNAR geneDepositorInsertshIFNAR1 Mmu (Ifnar1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSpuro shID3#1
Plasmid#19163DepositorInsertsmall hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDV05.1_pEF1a-iRFP720_frameshifted
Plasmid#200408PurposeExpresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent proteinDepositorInsertiRFP720 (frameshift mutant)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSpuro shID1#6
Plasmid#19165DepositorInsertsmall hairpin RNA against inhibitor of DNA binding 1 (ID1 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRShygro shID3#1
Plasmid#19164DepositorInsertsmall hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P shISCU
Plasmid#102972PurposeSuppression of ISCUDepositorInsertshISCU
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
SHIP2-ΔPR1
Plasmid#214902Purposeexpression of the truncated variants of the SHIP2 without first proline-rich domainDepositorInserthuman SHIP2 delta aa 123-410 (INPPL1 Human)
UseTagsV5/HisExpressionMammalianMutationdelta 123-410aaPromoterCMVAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-Washington
Plasmid#132758PurposeEncodes an iconic representation of a portrait of George WashingtonDepositorInsertWashington1
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shIrak1-2
Plasmid#180398PurposeProducing AAV that encodes mouse Irak1 shRNA-2 with miR-E backboneDepositorInsertshIrak1-2 (Irak1 Mouse)
UseAAV and RNAiTagsExpressionMutationPromoterCBAAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shIrak1-3
Plasmid#180399PurposeProducing AAV that encodes mouse Irak1 shRNA-3 with miR-E backboneDepositorInsertshIrak1-3 (Irak1 Mouse)
UseAAV and RNAiTagsExpressionMutationPromoterCBAAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shIrak1-1
Plasmid#180397PurposeProducing AAV that encodes mouse Irak1 shRNA-1 with miR-E backboneDepositorInsertshIrak1-1 (Irak1 Mouse)
UseAAV and RNAiTagsExpressionMutationPromoterCBAAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLIB_BSSHI_6xHisTEV-WIPI2d
Plasmid#190863PurposePlasmid for the expression of purification of WIPI2d: SMC Internal reference: SMC1216DepositorInsertWIPI2 (WIPI2 Human)
UseTagsExpressionBacterial and InsectMutationSNP C to G at position 4562 (Val)PromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24TEV-tSHIP1
Plasmid#183770PurposeExpresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and 6HIS tags from pET24 backboneDepositorInsertSHIP1 (INPP5D Human)
UseTags6HIS and Tobacco Etch Virus (TEV) Cleavage siteExpressionBacterialMutationincludes aa1 to aa883 onlyPromoterT7 lacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24TEV-tSHIP2
Plasmid#183771PurposeExpresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backboneDepositorInsertSHIP2 (INPPL1 Human)
UseTags6HIS and Tobacco Etch Virus (TEV) Cleavage siteExpressionBacterialMutationincludes aa18 to aa985 onlyPromoterT7 lacAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
frameshift ELL2
Plasmid#127257PurposeFor in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.DepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationXhoI site between the 1st &2nd Met was filled…PromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only