pET24TEV-tSHIP1
(Plasmid
#183770)
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PurposeExpresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and 6HIS tags from pET24 backbone
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 183770 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET24b
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Backbone manufacturerNovagen-EMD Millipore
- Backbone size w/o insert (bp) 5309
- Total vector size (bp) 7961
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Modifications to backboneTobacco Etch Virus (TEV) Cleavage Site inserted between HindIII and XhoI
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSHIP1
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Alt nametSHIP1
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Alt nameINPP5D
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SpeciesH. sapiens (human)
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Insert Size (bp)2656
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Mutationincludes aa1 to aa883 only
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GenBank IDNM_001017915
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Entrez GeneINPP5D (a.k.a. SHIP, SHIP-1, SHIP1, SIP-145, hp51CN, p150Ship)
- Promoter T7 lac
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Tags
/ Fusion Proteins
- Tobacco Etch Virus (TEV) Cleavage site (C terminal on backbone)
- 6HIS (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET24TEV-tSHIP1 was a gift from William Kerr (Addgene plasmid # 183770 ; http://n2t.net/addgene:183770 ; RRID:Addgene_183770) -
For your References section:
LRBA Deficiency Can Lead to Lethal Colitis That Is Diminished by SHIP1 Agonism. Sudan R, Fernandes S, Srivastava N, Pedicone C, Meyer ST, Chisholm JD, Engelman RW, Kerr WG. Front Immunol. 2022 May 4;13:830961. doi: 10.3389/fimmu.2022.830961. eCollection 2022. 10.3389/fimmu.2022.830961 PubMed 35603158