Cross-species genetic screens identify transglutaminase 5 as a regulator of polyglutamine-expanded ataxin-1.
Lee WS, Al-Ramahi I, Jeong HH, Jang Y, Lin T, Adamski CJ, Lavery LA, Rath S, Richman R, Bondar VV, Alcala E, Revelli JP, Orr HT, Liu Z, Botas J, Zoghbi HY
J Clin Invest. 2022 May 2;132(9). pii: 156616. doi: 10.1172/JCI156616. PubMed Article
Lee WS, Al-Ramahi I, Jeong HH, Jang Y, Lin T, Adamski CJ, Lavery LA, Rath S, Richman R, Bondar VV, Alcala E, Revelli JP, Orr HT, Liu Z, Botas J, Zoghbi HY
J Clin Invest. 2022 May 2;132(9). pii: 156616. doi: 10.1172/JCI156616. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 180391 | AAV.CBA.YFP.miR-E_shStk16-1 | Producing AAV that encodes mouse Stk16 shRNA-1 with miR-E backbone |
| 180392 | AAV.CBA.YFP.miR-E_shStk16-2 | Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone |
| 180393 | AAV.CBA.YFP.miR-E_shStk16-3 | Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone |
| 180394 | AAV.CBA.YFP.miR-E_shTgm5-1 | Producing AAV that encodes mouse Tgm5 shRNA-1 with miR-E backbone |
| 180395 | AAV.CBA.YFP.miR-E_shTgm5-2 | Producing AAV that encodes mouse Tgm5 shRNA-2 with miR-E backbone |
| 180396 | AAV.CBA.YFP.miR-E_shTgm5-3 | Producing AAV that encodes mouse Tgm5 shRNA-3 with miR-E backbone |
| 180397 | AAV.CBA.YFP.miR-E_shIrak1-1 | Producing AAV that encodes mouse Irak1 shRNA-1 with miR-E backbone |
| 180398 | AAV.CBA.YFP.miR-E_shIrak1-2 | Producing AAV that encodes mouse Irak1 shRNA-2 with miR-E backbone |
| 180399 | AAV.CBA.YFP.miR-E_shIrak1-3 | Producing AAV that encodes mouse Irak1 shRNA-3 with miR-E backbone |
| 180400 | AAV.CBA.YFP.miR-E_shSrpk3-1 | Producing AAV that encodes mouse Srpk3 shRNA-1 with miR-E backbone |
| 180401 | AAV.CBA.YFP.miR-E_shSrpk3-2 | Producing AAV that encodes mouse Srpk3 shRNA-2 with miR-E backbone |
| 180402 | AAV.CBA.YFP.miR-E_shSrpk3-3 | Producing AAV that encodes mouse Srpk3 shRNA-3 with miR-E backbone |
| 180403 | W118-1_hSTK16-Flag | lentiviral transduction of human STK16 (with a C-terminal Flag tag) gene |
| 180404 | W118-1_Flag-hSRPK3 | lentiviral transduction of human SRPK3 (with an N-terminal Flag tag) gene |
| 180405 | W118-1_Flag-hIRAK1 | lentiviral transduction of human IRAK1 (with an N-terminal Flag tag) gene |
| 180406 | W118-1_hTGM1-Flag | lentiviral transduction of human TGM1 (with a C-terminal Flag tag) gene |
| 180407 | W118-1_hTGM2-Flag | lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal Flag tag) gene |
| 180408 | W118-1_hTGM3-Flag | lentiviral transduction of human TGM3 (with a C-terminal Flag tag) gene |
| 180409 | W118-1_hTGM4-Flag | lentiviral transduction of human TGM4 (with a C-terminal Flag tag) gene |
| 180410 | W118-1_hTGM5-Flag | lentiviral transduction of human TGM5 (with a C-terminal Flag tag) gene |
| 180411 | W118-1_hTGM6-Flag | lentiviral transduction of human TGM6 (with a C-terminal Flag tag) gene |
| 180412 | W118-1_hTGM2_C277S-Flag | lentiviral transduction of human TGM2 transcript variant 2 with a point mutation (C277S) that inactivate the enzyme and also with a C-terminal Flag tag |
| 180413 | W118-1_hTGM2-V5 | lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal V5 tag) gene |
| 180414 | W118-1_hTGM5-V5 | lentiviral transduction of human TGM5 (with a C-terminal V5 tag) gene |