pET24TEV-tSHIP2
(Plasmid #183771)

• Purpose: Expresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backbone
• Depositing Lab: William Kerr
• Publication: Sudan et al Front Immunol. 2022 May 4;13:830961. doi: 10.3389/fimmu.2022.830961. eCollection 2022.

Backbone

• Vector backbone: pET24b
• Backbone manufacturer: Novagen-EMD Millipore
• Backbone size w/o insert (bp): 5309
• Total vector size (bp): 8219
• Modifications to backbone: Tobacco Etch Virus (TEV) Cleavage Site inserted between HindIII and XhoI
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SHIP2
• Alt name: tSHIP2
• Alt name: INPPL1
• Species: H. sapiens (human)
• Insert Size (bp): 2920
• Mutation: includes aa18 to aa985 only
• GenBank ID: NM_001567
• Entrez Gene: INPPL1 (a.k.a. OPSMD, SHIP2)
• Promoter: T7 lac
• Tags / Fusion Proteins:
  • Tobacco Etch Virus (TEV) Cleavage site (C terminal on backbone)
  • 6HIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry