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Plasmid#136583PurposeExpresses an inducible short hairpin targeting human JUND sequenceDepositorInsertshJUNDv2 (JUND Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLVX-EGFP-IRES-puro
Plasmid#128652Purposemammalian expression of EGFP with puromycin selectionDepositorInsertEGFP
UseLentiviralTagsN/AExpressionMammalianMutationWild typePromoterCMVAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro (PX459) V2.0
Plasmid#62988PurposeCas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorHas ServiceCloning Grade DNAInserthSpCas9-2A-Puro V2.0
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterCbhAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-GFP
Plasmid#171123PurposeDoxycycline inducible expression of GFPDepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-NGLY1-Puro
Plasmid#181916PurposeLentiviral plasmid that directs the expression of NGLY1 in mammalian cells.DepositorInsertN-glycanase 1 (NGLY1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRIPZ-bioGEF-UBnc-PURO
Plasmid#208044PurposeEnables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycinDepositorInsertUBnc (UBC Human)
UseLentiviralTagsLow-affinity AvitagExpressionMutationL73P Mutation near C-terminus suppresses cleavage…PromotertetO/UBCAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-dCasRx-ALKBH5-PURO
Plasmid#175582PurposeExpresses dCasRx-ALKBH5 in mammalian cellDepositorInsertdCasRx-ALKBH5
UseCRISPR and LentiviralTagsFLAG-HAExpressionMammalianMutationRfxCas13d R239A/H244A/R858A/H863APromoterpMSCVAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-myr-HA-Akt1
Plasmid#46969Purposelentiviral expression of myr-HA-Akt1DepositorInsertAKT1 (AKT1 Human)
UseLentiviralTagsHA and myrExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBOB-EF1-FastFUCCI-Puro
Plasmid#86849Purpose3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selectionDepositorInsertsmKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1 and PGKAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK232 (CMV-OsTIR1-PURO)
Plasmid#72834PurposeExpresses OsTIR1 under the control of the CMV promoterDepositorInsertAAVS1-CMV-OsTIR1-PURO-AAVS1
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-puro-Scrambled
Plasmid#47541PurposeTet inducible scrambled shRNA.DepositorInsertScrambled shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-CD19 puro
Plasmid#196634PurposeExpress CD19DepositorInsertCD19 (CD19 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO-sgRNA-puro
Plasmid#104321Purpose3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterH1/TOAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
MCS-BioID2-HA pBabe-puro
Plasmid#120308PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tagDepositorTypeEmpty backboneUseRetroviralTags-BioID2-HAExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Puro-P2A-EGFP
Plasmid#137729PurposeFor simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).DepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsEGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W
Plasmid#112925PurposeLentiviral vector for SAM-CRISPRa gRNA expression with puroBFPDepositorInserthU6 promoter, gRNA-SAM expression cassette
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST JNKKTRClover
Plasmid#59151PurposeLentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertJNK Kinase Translocation Reporter (MAPK8 Mouse, Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST p38KTRClover
Plasmid#59152PurposeLentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)DepositorInsertp38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV(W-)U6sgRNA5(BbsI)-PGKpuroBFP
Plasmid#102797PurposeRetrovirus for testing CRISPR KO activity (empty) - non-targeting control plasmid contains GFP instead of PuroRDepositorInsertssgRNA
EGFP
UseCRISPR and RetroviralTagsExpressionMammalianMutationH231LPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W
Plasmid#105034PurposeLentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFPDepositorInsertPtpn23 (Ptpn23 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Puro shRNA Scramble
Plasmid#162011PurposeLentiviral negative control vector containing scramble shRNADepositorInsertscramble shRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462) V2.0
Plasmid#62987PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro K-Ras V12
Plasmid#9052PurposeRetroviral expression of a constitutively active form of K-RasDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsExpressionMammalianMutationG12V. Constitutively active.PromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase
Plasmid#119816Purpose3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cellsDepositorInsertsLuciferase
Puromycin
UseLentiviral and LuciferaseTagsEGFP and V5ExpressionMammalianMutationStop codon removed in the frame of the V5 frame v…PromoterCMV/TO and Human Phosphoglycerate kinaseAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-NGN2-EMX1 puro-BFP
Plasmid#182312PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, puromycin selection, BFPUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV Puro DEST ERKKTRClover
Plasmid#59150PurposeLentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)DepositorInsertERK Kinase Translocation Reporter
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABE puro H-Ras V12
Plasmid#9051PurposeRetroviral expression of a constitutively active form of H-RasDepositorInsertHras V12 (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationG12V, constitutively activePromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV Puro DEST p38KTRmCerulean3
Plasmid#59155PurposeLentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)DepositorInsertp38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
UseLentiviralTagsmCerulean3ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-Puro (pRW1441)
Plasmid#216217PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-RAP1A-WT-puro
Plasmid#156166PurposeLentiviral vector for expression of RAP1A-WT, with puromycin selectionDepositorInsertRAP1A (RAP1A Human)
UseLentiviralTagsnoneExpressionMammalianMutationwild type formPromoterhuman PGKAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro-EGFP-FAK
Plasmid#38194DepositorInsertPTK2 protein tyrosine kinase 2 (Ptk2 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
iC13 PB-TO-oNgn2-BFP-puro
Plasmid#204733PurposeImproved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.InsertOptimized neurogenin-2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-p21
Plasmid#171122PurposeDoxycycline inducible expression of p21DepositorInsertp21 (CDKN1A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-puro-mGSDME D270A
Plasmid#223518PurposeExpresses mGSDME D270A in mammalian cellsDepositorInsertGSDME (Gsdme Mouse)
UseLentiviralTagsExpressionMammalianMutationD270APromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
p23-puro-EGFP-RPA194
Plasmid#179237Purposeoverexpression in human cellsDepositorInsertRPA194 (POLR1A Human)
UseTagsEGFPExpressionMammalianMutationPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Puro
Plasmid#85132Purposelentiviral backboneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP
Plasmid#102796PurposeRetrovirus for delivery of one sgRNA (empty, bbsI)DepositorInsertU6 sgRNA
UseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiCROP-PuroR-P2A-GFP-U6
Plasmid#200029PurposeLentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAsDepositorTypeEmpty backboneUseLentiviralTagsPuroR-P2A-GFPExpressionMammalianMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-Puro-TNNT2-Neo
Plasmid#214013PurposeExpression of cardiac troponin TDepositorInsertTNNT2 (TNNT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro EIF2AK1/HRI_sgRNA
Plasmid#218529PurposesgRNA targeting human EIF2AK1/HRIDepositorInsertEIF2AK1 (EIF2AK1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-puro-NLS-GFP
Plasmid#185473PurposeMammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.DepositorTypeEmpty backboneUseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
P3-dCas9-Tet1-GFP-Puro
Plasmid#190728PurposeExpression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)DepositorInsertdCas9-tet1
UseCRISPRTags3XFLAG and SV40 NLSExpressionMammalianMutationChanged Pro434 codon from CCG to CCC and Gly468 f…PromoterpCAGAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-5HRE-GFP PuroR
Plasmid#128958PurposeLentiviral plasmid for expression of hypoxia-responsive enhanced green fluorescent proteinDepositorInsert5X HRE of VEGF (VEGFA Human)
UseLentiviralTagsd2EGFPExpressionMammalianMutationPromoterminimal CMV promoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-Halo-hMRE11(wt)
Plasmid#208393PurposeTo generate stable cell line expressing Halo-human MRE11(WT) by Retroviral infection.DepositorInsertHuman MRE11 (MRE11 Human)
UseLuciferase and RetroviralTagsExpressionMammalianMutationH129NPromoterAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-K263E
Plasmid#133758PurposeLentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged lysine 263 to glutamate.PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA
Plasmid#218528PurposesgRNA targeting human EIF2AK4/GCN2DepositorInsertEIF2AK4 (EIF2AK4 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA
Plasmid#218666PurposesgRNA targeting human EIF2AK3/PERKDepositorInsertEIF2AK3 (EIF2AK3 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro GFP shRNA
Plasmid#10675DepositorInsertGFP shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP
Plasmid#119903PurposeBxb1 integration plasmid for cuO array with Puro selection cassetteDepositorInsertcuOx144 array
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV puro Tet VSVG
Plasmid#82724PurposeLentiviral plasmid that expresses VSVG protein under a doxycycline-inducible promoterDepositorInsertVSVG
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2016AvailabilityAcademic Institutions and Nonprofits only