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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 10675 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMKO.1
- Backbone size w/o insert (bp) 6700
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Vector typeMammalian Expression, Retroviral, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP shRNA
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Alt nameGFP
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Speciesjellyfish
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Insert Size (bp)58
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pLXSN 5' (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
shRNA oligo sequence: 5'-GCTACGTCCAGGAGCGCACCCTCGAGGGTGCGCTCCTGGACGTAGCC
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMKO.1 puro GFP shRNA was a gift from William Hahn (Addgene plasmid # 10675 ; http://n2t.net/addgene:10675 ; RRID:Addgene_10675) -
For your References section:
The telomerase reverse transcriptase regulates chromatin state and DNA damage responses. Masutomi K, Possemato R, Wong JM, Currier JL, Tothova Z, Manola JB, Ganesan S, Lansdorp PM, Collins K, Hahn WC. Proc Natl Acad Sci U S A. 2005 Jun 7. 102(23):8222-7. 10.1073/pnas.0503095102 PubMed 15928077