Skip to main content
Addgene

Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells.

Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, Betzig E, Meyer T
Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub 2016 Nov 14. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
85130pCAG-AMPH1-BAR-mCherryBAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry
85131pLV-Ftractin-mCherrylentiviral expression of an F-actin live-cell marker (mCherry)
85132pLV-EF1a-IRES-Purolentiviral backbone
85133pLV-EF1a-IRES-Blastlentiviral backbone
85134pLV-EF1a-IRES-Hygrolentiviral backbone
85139pLV-EF1a-IRES-Neolentiviral backbone
85140pLV-hTERT-IRES-hygroLentiviral expression of hTERT, used to create immortalized cell lines
85143pLV-CDH5-mCitrine-IRES-purolentiviral expression of human VE-cadherin, C-terminally tagged with mCitrine
85144pLV-CDH5-mRuby3-IRES-purolentiviral expression of human VE-cadherin, C-terminally tagged with mRuby3
85145pLV-mTurquoise-MLC-IRES-neolentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise
85146pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blastlentiviral expression of a stoichiometric F-actin and myosin light chain reporter
85152pCAG-mCherry-FKBP-GEF(ARHGEF1)acute activation of Rho through rapamycin-induced translocation to membrane-localized FRB
85156pCAG-mCherry-FKBP-GEF(TIAM1)acute activation of Rac through rapamycin-induced translocation to membrane-localized FRB

Antibodies from Article