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Purposelentiviral expression of myr-HA-Akt1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 46969 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePCDH-CMV-MCS-EF1-PURO
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Backbone manufacturerSystem Biosciences
- Backbone size w/o insert (bp) 7384
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAKT1
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Alt namev-akt murine thymoma viral oncogene homolog 1__
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SpeciesH. sapiens (human)
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Insert Size (bp)1500
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Entrez GeneAKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
- Promoter CMV
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Tags
/ Fusion Proteins
- myr (N terminal on insert)
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Myr-HA-Akt1 was amplified by PCR from pcDNA3-HA-Myr-Akt1 (Addgene plasmid #9008) and subcloned into the XbaI and EcoRI sites of the pCDH-CMV-MCS-EF1-Puro lentiviral vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDH-puro-myr-HA-Akt1 was a gift from Jialiang Wang (Addgene plasmid # 46969 ; http://n2t.net/addgene:46969 ; RRID:Addgene_46969) -
For your References section:
Inhibition of BET bromodomain targets genetically diverse glioblastoma. Cheng Z, Gong Y, Ma Y, Lu K, Lu X, Pierce LA, Thompson RC, Muller S, Knapp S, Wang J. Clin Cancer Res. 2013 Apr 1;19(7):1748-59. doi: 10.1158/1078-0432.CCR-12-3066. Epub 2013 Feb 12. 10.1158/1078-0432.CCR-12-3066 PubMed 23403638