Addgene

We narrowed to 2,249 results for: PRK

Showing: 1701 - 1720 of 2249 results

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HA-PRAS40 AA

Plasmid

#86759

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

human PRAS40 (AKT1S1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

AA (S183A, S221A)

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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1013 pcDNA3 T7 Akt1 T308A S473A

Plasmid

#9030

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 T308A S473A (AKT1 Human)

Use

Tags

Expression

Mammalian

Mutation

T308A S473A

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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myc-Atg14 (71-181)

Plasmid

#86753

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

atg14 (ATG14 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

aa 71-181

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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myc-Atg14 (1-181)

Plasmid

#86752

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

atg14 (ATG14 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

aa 1-181

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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myc-Atg14 (1-200)

Plasmid

#86750

Purpose

N-terminal myc-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

Atg14 (ATG14 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

aa 1-200

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-psMEK1

Plasmid

#89361

Purpose

A lenti-viral vector expressing photoswitchable MEK1 in mammalian cells

Depositor

Michael Lin

Article

Zhou et al Science. 2017 Feb 24;355(6327):836-842.

Insert

psMEK1 (MAP2K1 Human, Synthetic)

Use

Lentiviral

Tags

Human influenza hemagglutinin (HA) tag and pdDron…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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904 pLNCX HA Akt1 K179M

Plasmid

#9007

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 K179M (AKT1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

K179M. Kinase dead.

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA-Akt1-aa1-149

Plasmid

#73410

Purpose

Expresses HA tagged aa1-149 of AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 1-149 only of AKT1

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA-Akt1-aa120-433

Plasmid

#73411

Purpose

Expresses HA tagged aa120-433 AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 120-433 of AKT1

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
PH Akt-Venus

Plasmid

#85223

Purpose

Venus tagged PH domain of Akt for use as a PIP3 sensor

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Insert

PH AKT(1-148) (AKT1 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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myc-KIF5A

Plasmid

#127616

Purpose

N-terminal myc-tagged mouse KIF5A

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5A (Kif5a Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARF6-GFP

Plasmid

#67394

Purpose

Mammalian expression of hArf6 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-AktAR

Plasmid

#61624

Purpose

FRET-based biosensor for monitoring Akt activity

Depositor

Jin Zhang

Article

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR (AKT1 Human, Synthetic, Budding Yeast)

Use

Tags

Cerulean and cpVenus[E172]

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLpA-DN.JNK1

Plasmid

#51942

Purpose

Adenoviral vector for dominant negative JNK1 expression

Depositor

Jeffery Molkentin

Article

Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

Insert

DN JNK1 (Mapk8 Mouse)

Use

Adenoviral

Tags

FLAG

Expression

Mutation

dominant negative: the dual phosphorylation moti…

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pGEX6P1-HA-MEK5

Plasmid

#53166

Purpose

Expresses GST-HA tagged human MEK5 from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK5 (MAP2K5 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-Akt1-aa1-408

Plasmid

#73412

Purpose

Expresses HA tagged aa1-408 of AKT1

Depositor

Jie Chen

Article

Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

Insert

AKT1 (AKT1 Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

Expresses aa 1-408 of AKT1

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
1071 pBabe puroL myr Akt T308A S473A

Plasmid

#9014

Depositor

William Sellers

Article

DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

Insert

Akt1 T308A S473A (AKT1 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

T308A S473A

Promoter

Available since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only
783 pCScherryActMEK

Plasmid

#31880

Depositor

Nathan Lawson

Article

Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

Insert

MEK1 (MAP2K1 Human)

Use

Expression vector

Tags

mCherry

Expression

Mutation

Ser218Glu Ser222Asp; activated MEKK

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARF6-V5-His6

Plasmid

#67415

Purpose

Expression of hArf6 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL8B

Plasmid

#67467

Purpose

Mammalian expression of hArl8b with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8B (ARL8B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only