pcDNA3-AktAR
(Plasmid
#61624)
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PurposeFRET-based biosensor for monitoring Akt activity
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 61624 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5446
- Total vector size (bp) 7541
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAktAR
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SpeciesH. sapiens (human), S. cerevisiae (budding yeast), Synthetic
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Insert Size (bp)2007
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Entrez GeneAKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
- Promoter CMV
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Tags
/ Fusion Proteins
- Cerulean (N terminal on insert)
- cpVenus[E172] (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 forward
- 3′ sequencing primer BGH reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-AktAR was a gift from Jin Zhang (Addgene plasmid # 61624 ; http://n2t.net/addgene:61624 ; RRID:Addgene_61624) -
For your References section:
Spatiotemporal analysis of differential Akt regulation in plasma membrane microdomains. Gao X, Zhang J. Mol Biol Cell. 2008 Oct;19(10):4366-73. doi: 10.1091/mbc.E08-05-0449. Epub 2008 Aug 13. 10.1091/mbc.e08-05-0449 PubMed 18701703