pLpA-DN.JNK1
(Plasmid
#51942)
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PurposeAdenoviral vector for dominant negative JNK1 expression
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51942 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACCMVpLpA (pLpA)
- Backbone size w/o insert (bp) 8800
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Vector typeAdenoviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDN JNK1
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Alt nameMapk8
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Alt nameJNK1
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Alt namedominant negative JNK1
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SpeciesM. musculus (mouse)
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Mutationdominant negative: the dual phosphorylation motif TPY was changed to APF
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Entrez GeneMapk8 (a.k.a. JNK, JNK1, Prkm8, SAPK1)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLpA-DN.JNK1 was a gift from Jeffery Molkentin (Addgene plasmid # 51942 ; http://n2t.net/addgene:51942 ; RRID:Addgene_51942) -
For your References section:
c-Jun N-terminal kinases (JNK) antagonize cardiac growth through cross-talk with calcineurin-NFAT signaling. Liang Q, Bueno OF, Wilkins BJ, Kuan CY, Xia Y, Molkentin JD. EMBO J. 2003 Oct 1;22(19):5079-89. 10.1093/emboj/cdg474 PubMed 14517246