pCDNA3-HA-Akt1-aa1-149
(Plasmid
#73410)
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PurposeExpresses HA tagged aa1-149 of AKT1
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 73410 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCDNA3
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAKT1
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SpeciesH. sapiens (human)
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MutationExpresses aa 1-149 only of AKT1
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Entrez GeneAKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
- Promoter CMV
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Tag
/ Fusion Protein
- HA tag (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3-HA-Akt1-aa1-149 was a gift from Jie Chen (Addgene plasmid # 73410 ; http://n2t.net/addgene:73410 ; RRID:Addgene_73410) -
For your References section:
Lanthionine synthetase C-like protein 2 (LanCL2) is a novel regulator of Akt. Zeng M, van der Donk WA, Chen J. Mol Biol Cell. 2014 Dec 1;25(24):3954-61. doi: 10.1091/mbc.E14-01-0004. Epub 2014 Oct 1. 10.1091/mbc.E14-01-0004 PubMed 25273559