Showing: 121 - 140 of 12932 results
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Plasmid#51058PurposeSame as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.DepositorInsertRGR-GFP
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterYeast ADH1 promoterAvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP-mm
Plasmid#51059PurposeSame as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.DepositorInsertRGR-GFP
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterYeast ADH1 promoterAvailabilityAcademic Institutions and Nonprofits only -
pST1374-N-NLS-flag-linker-Cas9-H840A
Plasmid#51129PurposeCas9 nickase optimized for nuclear import.DepositorInsertCas9 nickase
UseCRISPRTagsFlag and NLSExpressionMammalianMutationH840APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pST1374-N-NLS-flag-linker-Cas9-D10A
Plasmid#51130PurposeCas9 nickase optimized for nuclear import.DepositorInsertCas9 nickase
UseCRISPRTagsFlag and NLSExpressionMammalianMutationD10A (nickase mutant)PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pUC57-sgRNA expression vector
Plasmid#51132PurposeFor in vitro transcription of sgRNA from the T7 promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK-puromycin
Plasmid#51133Purposeexpresses sgRNA from the U6 promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hCas9
Plasmid#51142PurposeExpresses hCas9 under the CAG promoter for CRISPRDepositorInserthCas9
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
MLM3636
Plasmid#43860Purposeguide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoterDepositorInsertNone
UseCRISPRTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
JDS246
Plasmid#43861PurposeExpresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promotersDepositorInsertmammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
UseCRISPRTags3X FLAGExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
p3s-Cas9HC
Plasmid#43945DepositorInsertCas9
UseCRISPRTagsHA epitope and NLSExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pdCas9-humanized
Plasmid#44246PurposeExpression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdownDepositorInsertdead Cas9 with 3X NLS
UseCRISPR and Retroviral; VectorTags3x NLSExpressionMammalianMutationD10A H840A (catalytically inactive)PromoterMSCV LTR promoterAvailabilityAcademic Institutions and Nonprofits only -
pdCas9::BFP-humanized
Plasmid#44247PurposeExpression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdownDepositorInsertdCas9 fused to BFP
UseCRISPR and RetroviralTags2xNLS & tagBFPExpressionMammalianMutationD10A H840A (catalytically inactive)PromoterMSCV LTR promoterAvailabilityAcademic Institutions and Nonprofits only -
pgRNA-humanized
Plasmid#44248PurposeExpression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdownDepositorInsertguide RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-bacteria
Plasmid#44249PurposeaTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdownDepositorInsertdCas9 (bacteria)
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterpLtetO-1AvailabilityAcademic Institutions and Nonprofits only -
pwtCas9-bacteria
Plasmid#44250PurposeaTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdownDepositorInsertwild-type Cas9
UseCRISPRTagsExpressionBacterialMutationPromoterpLtetO-1AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-bacteria
Plasmid#44251PurposeExpression of customizable guide RNA (gRNA) for bacterial gene knockdownDepositorInsertguide RNA (bacteria)
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR::rpsL
Plasmid#44505PurposeA crRNA expression plasmid specific to the rpsL allele.DepositorInsertCRISPR::rpsL (rpsL )
UseCRISPR; E.coliTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCas9_GFP
Plasmid#44719PurposeCo-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cellsDepositorInsertCas9-2A-GFP
UseCRISPRTags2A-GFPExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pCas9D10A_GFP
Plasmid#44720PurposeCo-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cellsDepositorInsertCas9D10A-2A-GFP
UseCRISPRTags2A-GFPExpressionMammalianMutationD10A mutation- nickase mutantPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pST1374-NLS-flag-linker-Cas9
Plasmid#44758DepositorInsertHuman codon optimized Cas9
UseCRISPRTags32 amino acid linker, Flag, and NLSExpressionMammalianMutationcodon-optimizedPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 121 - 140 of 12932 results