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Showing: 1 - 20 of 155380 results

  1. pMD2.G

    Plasmid
    #12259
    Purpose
    VSV-G envelope expressing plasmid
    Depositor
    Didier Trono
    Insert
    VSV G
    Use
    Lentiviral; Envelope
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12259-plasmid-map-sequence-id-443804

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  2. psPAX2

    Plasmid
    #12260
    Purpose
    2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Type
    Empty backbone
    Use
    Lentiviral; Packaging
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12260-plasmid-map-sequence-id-467229

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  3. pRSV-Rev

    Plasmid
    #12253
    Purpose
    3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)
    Depositor
    Didier Trono
    Article
    Dull et al J Virol. 1998 Nov . 72(11):8463-71.
    Insert
    Rev (rev )
    Use
    Lentiviral; Packaging
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 4, 2006
    Availability
    Academic Institutions and Nonprofits only
    12253-plasmid-map-sequence-id-443803

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  4. P25-pZE-SUMO-PbTTA

    Plasmid
    #204634
    Purpose
    ColE1 ori, KanR, TetR, Tet promoter with a codon optimized pbTTA gene bearing an N-terminal hexahistidine tag followed by a SUMO tag and a TEV protease cleavage site.
    Depositor
    Aditya Kunjapur
    Article
    Jones et al Commun Biol. 2023 Sep 11;6(1):929. doi:
    Insert
    His-SUMO-TEV-PbTTA
    Use
    Synthetic Biology
    Tags
    His Tag; SUMO Tag; TEV Protease Site
    Expression
    Bacterial
    Mutation
    Promoter
    PLtetO-1 promoter
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    204634-plasmid-map-sequence-id-414414

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  7. lentiCRISPR v2

    Plasmid
    #52961
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52961-plasmid-map-sequence-id-244694

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  9. pAAV-syn-flex-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234442
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Abhi et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-NGR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234442-plasmid-map-sequence-id-465693

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  14. pCMV-VSV-G

    Plasmid
    #8454
    Purpose
    Envelope protein for producing lentiviral and MuLV retroviral particles. Use in conjunction with a packaging vector such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral).
    Depositor
    Bob Weinberg
    Article
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    8454-plasmid-map-sequence-id-404044

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  16. lentiGuide-Puro

    Plasmid
    #52963
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Has Service
    Cloning Grade DNA
    Inserts
    S. pyogenes sgRNA cassette
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-a and hU6
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52963-plasmid-map-sequence-id-331247

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  20. pORTMAGE-3

    Plasmid
    #72678
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    72678-plasmid-map-sequence-id-198909

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Showing: 1 - 20 of 155380 results